Hybrid electrokinetic manipulation in high-conductivity media

Jian Gao; Mandy L. Y. Sin; Tingting Liu; Vincent Gau; Joseph C. Liao; Pak Kin Wong

doi:10.1039/C1LC20054B

Hybrid electrokinetic manipulation in high-conductivity media

Jian Gao,^a^b Mandy L. Y. Sin,^a Tingting Liu,^a Vincent Gau,^c Joseph C. Liao^d and Pak Kin Wong*^a

* Corresponding authors

^a Department of Aerospace and Mechanical Engineering, University of Arizona, Tucson, USA
E-mail: pak@email.arizona.edu
Fax: +1-520-621-8191
Tel: +1-520-626-2215

^b Department of Chemical Engineering, Shandong Polytechnic University, Jinan, China

^c GeneFluidics Inc, Monterey Park, USA

^d Department of Urology, Stanford University, Palo Alto, USA

Abstract

This study reports a hybrid electrokinetic technique for label-free manipulation of pathogenic bacteria in biological samples toward medical diagnostic applications. While most electrokinetic techniques only function in low-conductivity buffers, hybrid electrokinetics enables effective operation in high-conductivity samples, such as physiological fluids (∼1 S m⁻¹). The hybrid electrokinetic technique combines short-range electrophoresis and dielectrophoresis, and long-range AC electrothermal flow to improve its effectiveness. The major technical hurdle of electrode instability for manipulating high conductivity samples is tackled by using a Ti–Au–Ti sandwich electrode and a 3-parallel-electrode configuration is designed for continuous isolation of bacteria. The device operates directly with biological samples including urine and buffy coats. We show that pathogenic bacteria and biowarfare agents can be concentrated for over 3 orders of magnitude using hybrid electrokinetics.

Supplementary files

Publication details

The article was received on 19 Jan 2011, accepted on 25 Mar 2011 and first published on 12 Apr 2011

Article type: Paper

DOI: 10.1039/C1LC20054B

Citation: Lab Chip, 2011,11, 1770-1775

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Hybrid electrokinetic manipulation in high-conductivity media

J. Gao, M. L. Y. Sin, T. Liu, V. Gau, J. C. Liao and P. K. Wong, Lab Chip, 2011, 11, 1770
DOI: 10.1039/C1LC20054B
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Lab on a Chip

Hybrid electrokinetic manipulation in high-conductivity media

Abstract

Supplementary files

Publication details

Hybrid electrokinetic manipulation in high-conductivity media

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