We develop antimicrobial testing methods and standard operating procedures to measure the effectiveness of hard surface disinfectants against:
- Staphylococcus aureus.
- Pseudomonas aeruginosa.
- Salmonella choleraesuis.
- Mycobacterium bovis (BCG).
- Clostridium difficile.
- Viruses.
- Biofilm.
Methods are available for the following types of antimicrobial formulations:
- Liquid.
- Spray.
- Towelette.
Our standard operating procedures are strict interpretations of AOAC International Exit and ASTM International standard methods. Exit
Methods
| Method ID |
Title |
|---|
| MB-01-07 |
Biosafety in the Laboratory |
| MB-02-07 |
Tracking of Test Microorganisms |
| MB-03-06 |
Screening of Polished Stainless Steel Cylinders, Porcelain Cylinders and Glass Slide Carriers Used in Disinfectant Efficacy Testing |
| MB-05-13 |
AOAC Use Dilution Method for Testing Disinfectants |
| MB-06-08 |
Germicidal Spray Products as Disinfectants (GSPT): Testing of Staphylococcus aureus, Pseudomonas aeruginosa, and Salmonella enterica |
| MB-07-06 |
Tuberculocidal Activity of Disinfectants: II. Confirmative in vitro Test for Determining Tuberculocidal Activity |
| MB-09-05 |
Disinfectant Towelette Test Against Staphylococcus aureus, Pseudomonas aeruginosa, and Salmonella enterica |
| MB-10-05 |
Media and Reagents Preparation and Quality Evaluation |
| MB-11-04 |
Neutralization Confirmation Assay for Disinfectant Products Tested against Mycobacterium bovis (BCG) |
| MB-13-04 |
Handling Spills of Biohazardous Materials |
| MB-15-03 |
Sporicidal Activity of Disinfectants Test (Bacillus × Porcelain component only) |
| MB-16-02 |
Quantitative Suspension Test Method for Determining Tuberculocidal Efficacy of Disinfectants against Mycobacterium bovis (BCG) |
| MB-17-03 |
Neutralization Confirmation Procedures for the AOAC Use-dilution method (UDM), the AOAC Germicidal Spray Products as Disinfectants Test (GSPT) and the Disinfectant Towelette Test (DTT) |
| MB-19-02 |
Growing a Pseudomonas aeruginosa Biofilm using the CDC Biofilm Reactor |
| MB-20-01 |
Single Tube Method for Measuring Disinfectant Efficacy Against Biofilm Grown in the CDC Biofilm Reactor |
| MB-21-02 |
Quantitative Three Step Method for Measuring the Efficacy of Liquid Sporicides against Spores of Bacillus subtilis on Hard Non-porous and Porous Surfaces |
| MB-22-03 |
Disinfectant Product Preparation and Sampling Procedures |
| MB-23-02 |
Disinfectant Towelette Test: Testing of Mycobacterium bovis (BCG) |
| MB-24-02 |
Testing of Mycobacterium bovis (BCG) Using the Germicidal Spray Products as Disinfectants Test |
| MB-25-02 |
OECD Quantitative Method for Evaluating Bactericidal Activity of Microbicides Used on Hard, Non-Porous Surfaces |
| MB-26-01 |
Neutralization of Microbicidal Activity using the OECD Quantitative Method for Evaluating Bactericidal Activity of Microbicides Used on Hard, Non-Porous Surfaces |
| MB-27-01 |
Germicidal and Detergent Sanitizing Action of Disinfectants Test |
| MB-28-04 |
Production of Spores of Clostridium difficile for Use in the Efficacy Evaluation of Antimicrobial Agents |
| MB-30-00 |
Preparation of hard water and other diluents for antimicrobial products |
| MB-31-03 |
Quantitative Disk Carrier Test Method (QCT-2) Modified for Testing Antimicrobial Products Against Spores of Clostridium difficile (ATCC 43598) on Inanimate, Hard, Non-porous Surfaces |
| MB-32-00 |
Quantitative Petri Plate Method (QPM) for Determining the Effectiveness of Antimicrobial Towelettes Against Spores of Clostridium difficile (ATCC 43598) on Inanimate, Hard Non-porous Surfaces |
| MB-33-00 |
Quantitative Petri Plate Method (QPM) for Determining the Effectiveness of Antimicrobial Towelettes Against Vegetative Bacteria on Inanimate, Hard, Non-porous Surfaces |
Procedures
The following standard operating procedures are pertinent to specific equipment and practices used in the EPA pesticide program's Microbiology Laboratory. The SOPs describe the laboratory's standard practices for:
- Calibrating equipment (EQ).
- Performing quality control procedures (QC).
- Administrative procedures (ADM).
- Maintaining chain of custody of samples (COC).
- Assuring quality management practices (QA).
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