Image: Laura Persat, Lausanne, Switzerland. Cover design: Erin Dewalt

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Featured Content

  • Article |

    Ubiquitous mammalian enzymes can scavenge uracil analogs, leading to non-specific background in cell-type-specific RNA labeling. This work reveals the enzymes involved and describes the uridine/cytidine kinase 2 and 2′-azidouridine pair as a highly specific and non-toxic alternative.

    • Sarah Nainar
    • , Bonnie J. Cuthbert
    • , Nathan M. Lim
    • , Whitney E. England
    • , Ke Ke
    • , Kanika Sophal
    • , Robert Quechol
    • , David L. Mobley
    • , Celia W. Goulding
    •  & Robert C. Spitale

  • Perspective
    | Open Access

    This Perspective describes the development and capabilities of SciPy 1.0, an open source scientific computing library for the Python programming language.

    • Pauli Virtanen
    • , Ralf Gommers
    • , Travis E. Oliphant
    • , Matt Haberland
    • , Tyler Reddy
    • , David Cournapeau
    • , Evgeni Burovski
    • , Pearu Peterson
    • , Warren Weckesser
    • , Jonathan Bright
    • , Stéfan J. van der Walt
    • , Matthew Brett
    • , Joshua Wilson
    • , K. Jarrod Millman
    • , Nikolay Mayorov
    • , Andrew R. J. Nelson
    • , Eric Jones
    • , Robert Kern
    • , Eric Larson
    • , C J Carey
    • , İlhan Polat
    • , Yu Feng
    • , Eric W. Moore
    • , Jake VanderPlas
    • , Denis Laxalde
    • , Josef Perktold
    • , Robert Cimrman
    • , Ian Henriksen
    • , E. A. Quintero
    • , Charles R. Harris
    • , Anne M. Archibald
    • , Antônio H. Ribeiro
    • , Fabian Pedregosa
    • , Paul van Mulbregt
    • , Aditya Vijaykumar
    • , Alessandro Pietro Bardelli
    • , Alex Rothberg
    • , Andreas Hilboll
    • , Andreas Kloeckner
    • , Anthony Scopatz
    • , Antony Lee
    • , Ariel Rokem
    • , C. Nathan Woods
    • , Chad Fulton
    • , Charles Masson
    • , Christian Häggström
    • , Clark Fitzgerald
    • , David A. Nicholson
    • , David R. Hagen
    • , Dmitrii V. Pasechnik
    • , Emanuele Olivetti
    • , Eric Martin
    • , Eric Wieser
    • , Fabrice Silva
    • , Felix Lenders
    • , Florian Wilhelm
    • , G. Young
    • , Gavin A. Price
    • , Gert-Ludwig Ingold
    • , Gregory E. Allen
    • , Gregory R. Lee
    • , Hervé Audren
    • , Irvin Probst
    • , Jörg P. Dietrich
    • , Jacob Silterra
    • , James T Webber
    • , Janko Slavič
    • , Joel Nothman
    • , Johannes Buchner
    • , Johannes Kulick
    • , Johannes L. Schönberger
    • , José Vinícius de Miranda Cardoso
    • , Joscha Reimer
    • , Joseph Harrington
    • , Juan Luis Cano Rodríguez
    • , Juan Nunez-Iglesias
    • , Justin Kuczynski
    • , Kevin Tritz
    • , Martin Thoma
    • , Matthew Newville
    • , Matthias Kümmerer
    • , Maximilian Bolingbroke
    • , Michael Tartre
    • , Mikhail Pak
    • , Nathaniel J. Smith
    • , Nikolai Nowaczyk
    • , Nikolay Shebanov
    • , Oleksandr Pavlyk
    • , Per A. Brodtkorb
    • , Perry Lee
    • , Robert T. McGibbon
    • , Roman Feldbauer
    • , Sam Lewis
    • , Sam Tygier
    • , Scott Sievert
    • , Sebastiano Vigna
    • , Stefan Peterson
    • , Surhud More
    • , Tadeusz Pudlik
    • , Takuya Oshima
    • , Thomas J. Pingel
    • , Thomas P. Robitaille
    • , Thomas Spura
    • , Thouis R. Jones
    • , Tim Cera
    • , Tim Leslie
    • , Tiziano Zito
    • , Tom Krauss
    • , Utkarsh Upadhyay
    • , Yaroslav O. Halchenko
    •  & Yoshiki Vázquez-Baeza

Current Issue |

Volume 17 Issue 2

Latest Research

  • Article |

    Ubiquitous mammalian enzymes can scavenge uracil analogs, leading to non-specific background in cell-type-specific RNA labeling. This work reveals the enzymes involved and describes the uridine/cytidine kinase 2 and 2′-azidouridine pair as a highly specific and non-toxic alternative.

    • Sarah Nainar
    • , Bonnie J. Cuthbert
    • , Nathan M. Lim
    • , Whitney E. England
    • , Ke Ke
    • , Kanika Sophal
    • , Robert Quechol
    • , David L. Mobley
    • , Celia W. Goulding
    •  & Robert C. Spitale

  • Perspective
    | Open Access

    This Perspective describes the development and capabilities of SciPy 1.0, an open source scientific computing library for the Python programming language.

    • Pauli Virtanen
    • , Ralf Gommers
    • , Travis E. Oliphant
    • , Matt Haberland
    • , Tyler Reddy
    • , David Cournapeau
    • , Evgeni Burovski
    • , Pearu Peterson
    • , Warren Weckesser
    • , Jonathan Bright
    • , Stéfan J. van der Walt
    • , Matthew Brett
    • , Joshua Wilson
    • , K. Jarrod Millman
    • , Nikolay Mayorov
    • , Andrew R. J. Nelson
    • , Eric Jones
    • , Robert Kern
    • , Eric Larson
    • , C J Carey
    • , İlhan Polat
    • , Yu Feng
    • , Eric W. Moore
    • , Jake VanderPlas
    • , Denis Laxalde
    • , Josef Perktold
    • , Robert Cimrman
    • , Ian Henriksen
    • , E. A. Quintero
    • , Charles R. Harris
    • , Anne M. Archibald
    • , Antônio H. Ribeiro
    • , Fabian Pedregosa
    • , Paul van Mulbregt
    • , Aditya Vijaykumar
    • , Alessandro Pietro Bardelli
    • , Alex Rothberg
    • , Andreas Hilboll
    • , Andreas Kloeckner
    • , Anthony Scopatz
    • , Antony Lee
    • , Ariel Rokem
    • , C. Nathan Woods
    • , Chad Fulton
    • , Charles Masson
    • , Christian Häggström
    • , Clark Fitzgerald
    • , David A. Nicholson
    • , David R. Hagen
    • , Dmitrii V. Pasechnik
    • , Emanuele Olivetti
    • , Eric Martin
    • , Eric Wieser
    • , Fabrice Silva
    • , Felix Lenders
    • , Florian Wilhelm
    • , G. Young
    • , Gavin A. Price
    • , Gert-Ludwig Ingold
    • , Gregory E. Allen
    • , Gregory R. Lee
    • , Hervé Audren
    • , Irvin Probst
    • , Jörg P. Dietrich
    • , Jacob Silterra
    • , James T Webber
    • , Janko Slavič
    • , Joel Nothman
    • , Johannes Buchner
    • , Johannes Kulick
    • , Johannes L. Schönberger
    • , José Vinícius de Miranda Cardoso
    • , Joscha Reimer
    • , Joseph Harrington
    • , Juan Luis Cano Rodríguez
    • , Juan Nunez-Iglesias
    • , Justin Kuczynski
    • , Kevin Tritz
    • , Martin Thoma
    • , Matthew Newville
    • , Matthias Kümmerer
    • , Maximilian Bolingbroke
    • , Michael Tartre
    • , Mikhail Pak
    • , Nathaniel J. Smith
    • , Nikolai Nowaczyk
    • , Nikolay Shebanov
    • , Oleksandr Pavlyk
    • , Per A. Brodtkorb
    • , Perry Lee
    • , Robert T. McGibbon
    • , Roman Feldbauer
    • , Sam Lewis
    • , Sam Tygier
    • , Scott Sievert
    • , Sebastiano Vigna
    • , Stefan Peterson
    • , Surhud More
    • , Tadeusz Pudlik
    • , Takuya Oshima
    • , Thomas J. Pingel
    • , Thomas P. Robitaille
    • , Thomas Spura
    • , Thouis R. Jones
    • , Tim Cera
    • , Tim Leslie
    • , Tiziano Zito
    • , Tom Krauss
    • , Utkarsh Upadhyay
    • , Yaroslav O. Halchenko
    •  & Yoshiki Vázquez-Baeza

  • Article |

    Phenotypic earth mover’s distance (PhEMD) facilitates the comparison of single-cell experimental conditions, each of which is a high-dimensional dataset, and identifies axes of variation among multicellular biospecimens.

    • William S. Chen
    • , Nevena Zivanovic
    • , David van Dijk
    • , Guy Wolf
    • , Bernd Bodenmiller
    •  & Smita Krishnaswamy

  • Article |

    Advances in MINFLUX nanoscopy enable multicolor imaging over large fields of view, bringing true nanometer-scale fluorescence imaging to labeled structures in fixed and living cells.

    • Klaus C. Gwosch
    • , Jasmin K. Pape
    • , Francisco Balzarotti
    • , Philipp Hoess
    • , Jan Ellenberg
    • , Jonas Ries
    •  & Stefan W. Hell
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Expanding the CRISPR Toolbox

Expanding the CRISPR Toolbox

The CRISPR-Cas9 system is best known for its ability to knock out or replace specific genes, via targeted cleavage of the genome. But scientists are developing many more applications, typically by using an inactive Cas9 to target other enzymes to specific genomic sites. From transcriptional regulation to base editing, these developments are extending the range of biological questions that can be probed with CRISPR/Cas9.
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