Locus Overview
- Standard Name
- CDC28 1 2
- Systematic Name
- YBR160W
- SGD ID
- S000000364
- Aliases
- CDK1 , HSL5 , SRM5
- Feature Type
- ORF , Verified
- Description
- Cyclin-dependent kinase (CDK) catalytic subunit; master regulator of mitotic and meiotic cell cycles; alternately associates with G1, S, G2/M phase cyclins, which provide substrate specificity; regulates basal transcription, chromosome dynamics, growth, morphogenesis; transcript induction in osmostress involves antisense RNA; human homologs CDK1, CDK2, CDK3 can each complement yeast cdc28 thermosensitive mutants; human CDK1, CDK2 can each complement yeast cdc28 null mutant 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
- Name Description
- Cell Division Cycle 18
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
ATCC/WashU Clones | BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Fungal Alignment | Synteny Viewer
S288C vs. other strains
Protein
Basic information about the protein. Click "Protein Details" for further information about the protein such as abundance data, domains, shared domains with other proteins, protein sequence retrieval for various strains, sequence-based physico-chemical proterties, protein modification sites sites, and external identifiers for the protein.
- Length (a.a.)
- 298
- Mol. Weight (Da)
- 34064.8
- Isoelectric Point
- 8.55
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Protein serine/threonine kinase subunit of multiple cyclin-dependent kinase (CDK) complexes, which regulate the mitotic and meiotic cell cycles as well as many other cellular processes. CDK complexes function at various locations throughout the cell
View computational annotations
Molecular Function
- Manually Curated
- High-Throughput
Biological Process
- Manually Curated
- 7-methylguanosine mRNA capping (IMP)
- meiotic DNA double-strand break processing (IGI)
- mitotic sister chromatid biorientation (IGI)
- negative regulation of double-strand break repair via nonhomologous end joining (IMP)
- negative regulation of meiotic cell cycle (IMP)
- negative regulation of mitotic cell cycle (IDA)
- negative regulation of sister chromatid cohesion (IMP)
- negative regulation of transcription from RNA polymerase II promoter during mitosis (IMP)
- negative regulation of transcription, DNA-templated (IMP, IDA)
- peptidyl-serine phosphorylation (IDA)
- phosphorylation of RNA polymerase II C-terminal domain (IDA)
- positive regulation of meiotic cell cycle (IDA, IMP)
- positive regulation of mitotic cell cycle (IMP)
- positive regulation of nuclear cell cycle DNA replication (IMP, IDA)
- positive regulation of spindle pole body separation (IGI, IMP)
- positive regulation of transcription from RNA polymerase II promoter (IMP)
- positive regulation of transcription, DNA-templated (IDA, IGI)
- positive regulation of triglyceride catabolic process (IGI, IMP)
- protein localization to nuclear periphery (IMP)
- protein localization to nucleus (IMP)
- protein phosphorylation involved in cellular protein catabolic process (IGI, IMP)
- protein phosphorylation involved in DNA double-strand break processing (IMP)
- protein phosphorylation involved in double-strand break repair via nonhomologous end joining (IMP)
- protein phosphorylation involved in mitotic spindle assembly (IGI)
- protein phosphorylation involved in protein localization to spindle microtubule (IMP)
- regulation of budding cell apical bud growth (IGI, IMP)
- regulation of double-strand break repair via homologous recombination (IMP)
- regulation of filamentous growth (IMP)
- regulation of nucleosome density (IMP)
- regulation of spindle assembly (IMP)
- regulation of telomere maintenance via telomerase (IGI)
- synaptonemal complex assembly (IMP)
- vesicle-mediated transport (IMP)
- High-Throughput
Cellular Component
- Manually Curated
- colocalizes with astral microtubule (IDA)
- cellular bud neck (IDA)
- cyclin-dependent protein kinase holoenzyme complex (IDA)
- cytoplasm (IDA)
- endoplasmic reticulum (IDA)
- nucleus (IDA)
- colocalizes with spindle pole body (IDA)
- High-Throughput
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Essential gene; conditional mutants display allele specific cell cycle arrest as either large, unbudded, conjugation-competent cells with a single, undivided nucleus and pear-shaped morphology at START in the G1 phase or as abnormally large, elongated cells with undivided nuclei at the G2/M phase transition; reduction-of-function mutant has a decreased rate of growth and buds in a bipolar or unipolar manner; conditional mutants are benomyl sensitive, have an elevated rate of plasmid and chromosome loss, and accumulate trigylcerides; homozygous diploid mutants have a sporulation defect
Classical Genetics
- conditional
- cell cycle passage through START: arrested
- cell cycle passage through START: normal
- cell cycle progression in G1 phase: arrested
- cell cycle progression through the G2/M phase transition: arrested
- cell cycle progression: arrested
- cell shape: abnormal
- cellular morphology: abnormal
- chromosome/plasmid maintenance: decreased
- heat sensitivity: increased
- protein/peptide distribution: abnormal
- protein/peptide modification: absent
- protein/peptide modification: decreased
- sporulation: decreased
- triglyceride accumulation: increased
- overexpression
- unspecified
- reduction of function
- conditional
- repressible
- null
- overexpression
- reduction of function
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
1065 total interactions for 576 unique genes
Physical Interactions
- Affinity Capture-MS: 70
- Affinity Capture-RNA: 2
- Affinity Capture-Western: 60
- Biochemical Activity: 427
- Co-localization: 4
- Co-purification: 9
- PCA: 46
- Protein-peptide: 2
- Reconstituted Complex: 28
- Two-hybrid: 36
Genetic Interactions
- Dosage Growth Defect: 6
- Dosage Lethality: 3
- Dosage Rescue: 25
- Negative Genetic: 41
- Phenotypic Enhancement: 54
- Phenotypic Suppression: 18
- Positive Genetic: 5
- Synthetic Growth Defect: 162
- Synthetic Lethality: 37
- Synthetic Rescue: 30
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 3
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2006-08-09
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.
