|
CFP/YFP/HcRed-3X-A Fluorescence Filter Set – Fluorophores |
| Table of fluorophores that may be used with the BrightLine® CFP/YFP/HcRed-3X-A filter set: Note that results may vary depending upon instrumentation and conditions. |
| Cyan Fluorophore | Peak
Excitation Wavelength* (nm) |
Peak
Emission Wavelength* (nm) |
| 7-hydroxy-4-methylcoumarin | 360 |
449 |
| ABQ | 344 |
445 |
| ACMA (9-amino-6-chloro-2-methoxyacridine) | 421 |
473 |
| Acridine | 362 |
462 |
| Acriflavin Feulgen SITSA | 390 |
460 |
| AMC, AMCA-S | 345 |
445 |
| AmCyan | 454 |
488 |
| Anthrocyl Stearate | 370 |
446 |
| Astrazon Yellow 7 GLL | 450 |
480 |
| Atabrine | 436 |
490 |
| Beta Lactamase | 409 |
450 |
| BFP (Blue Fluorescent Protein) | 382 |
448 |
| Bisbenzimide (Hoechst) | 360 |
461 |
| Blancophor FFG | 390 |
470 |
| BOBO-1, BO-PRO-1 | 462 |
481 |
| Catecholamine | 410 |
470 |
| CFP (Cyan Fluorescent Protein) | 434 |
477 |
| Chromomycin A | 436 |
470 |
| Coelenterazine Ca2+ Dye, bioluminescence | 429 |
465 |
| Coumarin | 360 |
449 |
| Coumarin Phalloidin | 387 |
470 |
| CPM Methylcoumarin | 384 |
469 |
| Dabcyl | 453 |
453 |
| DAPI | 359 |
461 |
| EBFP | 383 |
440 |
| ECFP | 434 |
477 |
| Genacryl Brilliant Yellow 10GF | 430 |
485 |
| Genacryl Yellow 5GF | 430 |
475 |
| Gloxalic Acid | 405 |
460 |
| Hoechst 33258 | 345 |
461 |
| Hoechst 33342 | 343 |
483 |
| HPTS | 355 |
465 |
| Laurodan | 355 |
460 |
| Leucophor SF, WS | 370 |
430 |
| Marina Blue | 365 |
460 |
| Mitramycin | 450 |
470 |
| Monochlorobimane | 380 |
461 |
| Pacific Blue™ | 405 |
455 |
| POPO-1 | 433 |
457 |
| PO-PRO-1 | 435 |
455 |
| Sevron Yellow L | 430 |
490 |
| sgBFP™ | 387 |
450 |
| SITS (Primuline) | 410 |
450 |
| SITS (Stilbene Isothiosulphonic Acid) | 365 |
460 |
| SpectrumAqua | 433 |
480 |
| SYTO 41 | 430 |
454 |
| SYTO 42 | 433 |
460 |
| SYTO 43 | 436 |
467 |
| SYTO 44 | 446 |
471 |
| SYTO Blue Fluorescent Nucleic Acid Stains 40, 41, 42, 43 | 428 |
454 |
| SYTO Blue Fluorescent Nucleic Acid Stain 44 | 446 |
471 |
| SYTO Blue Fluorescent Nucleic Acid Stain 45 | 452 |
484 |
| SYTOX Blue | 445 |
470 |
| Thioflavin TCN | 350 |
460 |
| Thiozole Orange | 453 |
480 |
| Y66W | 436 |
485 |
|
| Red Fluorophore | Peak
Excitation Wavelength* (nm) |
Peak
Emission Wavelength* (nm) |
| 5-Carboxynapthofluorescein (pH 10) | 555 |
615 |
| 5-ROX (carboxy-X-rhodamine) | 578 |
605 |
| 7-AAD | 546 |
647 |
| 7-Aminoactinomycin D (7-AAD) | 546 |
647 |
| Acid Fuchsin | 540 |
630 |
| Acridine Orange + RNA | 460 |
650 |
| Acridine Red | 528 |
620 |
| Alexa 568 | 577 |
603 |
| Alexa 594 | 594 |
618 |
| Alexa 633 | 632 |
649 |
| Alexa Fluor 568™ | 577 |
603 |
| Alexa Fluor 594™ | 590 |
617 |
| Alexa Fluor 633™ | 632 |
650 |
| Bodipy 630 | 625 |
642 |
| Bodipy 630/650-X | 625 |
642 |
| Bodipy TR | 589 |
617 |
| Bodipy TR ATP | 591 |
620 |
| Bodipy TR-X dye | 589 |
617 |
| Bodipy TR-X SE | 588 |
616 |
| Calcein/Ethidium Homodimer | 528 |
617 |
| Calcium Crimson™ Indicator | 590 |
615 |
| Calcium Crimson™ | 588 |
611 |
| Chlorophyll | 480 |
650 |
| Cy3.5™ | 580 |
591 |
| DDAO | 463 |
607 |
| Di-8-ANEPPS (non-ratio) | 488 |
605 |
| Di-8-ANEPPS, Di-4-ANEPPS | 468 |
635 |
| Ethidium Bromide | 523 |
595 |
| Ethidium Bromide § | 518 |
605 |
| Ethidium Homodimer-1 (EthD-1) | 528 |
617 |
| Feulgen (Pararosaniline) | 570 |
625 |
| Flazo Orange | 452 |
612 |
| FM 4-46 | 515 |
640 |
| HcRed | 590 |
614 |
| LDS 751 (RNA) | 590 |
607 |
| LightCycler 640 | 625 |
640 |
| Malachite Green | 628 |
650 |
| MitoTracker™ Red | 578 |
599 |
| mRFPCherry | 587 |
610 |
| Nile Red | 549 |
628 |
| Pararosaniline (Feulgen) | 570 |
625 |
| PE-Texas Red (Red 613) | 488 |
613 |
| PhotoResist | 365 |
610 |
| Pontochrome Blue Black | 545 |
605 |
| Propidium Iodide (PI) | 536 |
617 |
| Pyronine B | 565 |
605 |
| Red 613 | 565 |
613 |
| Red 613 (PE-Texas Red) | 488 |
613 |
| RH 414 | 500 |
635 |
| Rhodamine B | 540 |
625 |
| Rhodamine B extra | 550 |
605 |
| ROX | 585 |
605 |
| SNARF (carboxy) 514 Excitation pH 9 | 576 |
640 |
| SpectrumRed | 587 |
612 |
| SYTO 17 | 621 |
634 |
| SYTO 59 | 622 |
645 |
| SYTO 61 | 628 |
645 |
| SYTO 64 | 599 |
619 |
| SYTO Red Fluorescent Nucleic Acid Stains 17, 59, 61, 64 § | 615 |
632 |
| Texas Red® | 595 |
615 |
| Texas Red-X® Conjugate | 595 |
615 |
| Thiazine Red R | 596 |
615 |
| WW 781 | 605 |
639 |
| X-Rhodamine | 580 |
605 |
| YO-PRO-3 | 613 |
629 |
| YOYO-3 | 612 |
631 |
| YOYO-3, YO-PRO-3 § | 612 |
631 |
| * These are typical wavelengths to be used for guidance purposes only. Actual peak wavelengths may vary for different applications. |