The Human Immune Monitoring Center (HIMC) is designed to be a comprehensive source for immune monitoring services for clinical and translational studies.
Goals of the HIMC include:
- To provide standardized, state-of-the art immune monitoring assays at the RNA, protein, and cellular level
- To test and develop new technologies for immune monitoring
- To efficiently archive, report, and mine data from immune monitoring studies, so as to increase the value of the data and to assist in biomarker discovery
Description of Services
- Analysis of clinical samples by: multiplexed immunoassays (Luminex and MesoScale Discovery)
- Flow cytometry immunophenotyping, phosphoepitope analysis, and intracellular cytokine staining, by fluorescence and mass cytometry (CyTOF);genomic assays including microfluidic qPCR (Fluidigm BioMark)
- Genomic assays including microfluidic qPCR (Fluidigm BioMark)
Selected References
Nanofluidic proteomic assay for serial analysis of oncoprotein activation in clinical specimens. Fan AC, Deb-Basu D, Orban MW, Gotlib JR, Natkunam Y, O'Neill R, Padua RA, Xu L, Taketa D, Shirer AE, Beer S, Yee AX, Voehringer DW and Felsher DW. 2009. Nature Medicine 15:566-571. PubMedID: 19363496.
T helper type 1 and 17 cells determine efficacy of interferon-beta in multiple sclerosis and experimental encephalomyelitis. Axtell RC, de Jong BA, Boniface K, van der Voort LF, Bhat R, De Sarno P, Naves R, Han M, Zhong F, Castellanos JG, Mair R, Christakos A, Kolkowitz I, Katz L, Killestein J, Polman CH, de Waal Malefyt R, Steinman L, and Raman C. 2010. Nature Medicine 16:406-412. PubMedID: 20348925; PMCID: PMC3042885
Cell type-specific gene expression differences in complex tissues. Shen-Orr SS, Tibshirani R, Khatri P, Bodian DL, Staedtler F, Perry NM, Hastie T, Sarwal MM, Davis MM, and Butte AJ. 2010. Nature Methods 7:287-289. PubMedID: 20208531.
