Partly supported by funds from Stanford Cancer Institute, the Stanford Transgenic Mouse Research Facility is set to produce your transgenic and knockout mouse models. The facility provides low-cost DNA microinjection services, ES cell manipulation, and microinjection services to all Stanford as well as external investigators. The facility also provides services on timed pregnant females, strain rederivation, embryo and sperm cryopreservation, and IVF procedures for resurrecting frozen sperm.
Description of Services
- DNA Microinjection
- Site-specific gene knockin using integrase
- ES Cell Microinjection
- ES Cell Manipulation
- ES, karyotyping and MEF feeder cells
- Sperm Cryopreservation & IVF
- Embryo cryopreservation
- Mouse rederivation for SIM1 barrier facility
- DNA vector construction
- Human ESC/iPSC Services
- Preclinical Oncology Laboratory
Selected References
Site-specific recombinase strategy to create induced pluripotent stem cells efficiently with plasmid DNA. Karow M, Chavez CL, Farruggio AP, Geisinger JM, Keravala A, Jung WE, Lan F, Wu JC, Chen-Tsai Y, Calos MP. Stem Cells. 2011 Nov;29(11):1696-704. PubMedID: 21898697.
Cortical representations of olfactory input by trans-synaptic tracing. Miyamichi K, Amat F, Moussavi F, Wang C, Wickersham I, Wall NR, Taniguchi H, Tasic B, Huang ZJ, He Z, Callaway EM, Horowitz MA, Luo L. Nature. 2011 Apr 14;472(7342):191-6. PubMedID: 21179085; PMCID: PMC3073090
Embryonic stem cells require Wnt proteins to prevent differentiation to epiblast stem cells. ten Berge D, Kurek D, Blauwkamp T, Koole W, Maas A, Eroglu E, Siu RK, Nusse R. Nat Cell Biol. 2011 Aug 14;13(9):1070-5. PubMedID: 21841791.
