Bio

Immunology PhD candidate in Dr. Ravi Majeti lab; looking at AML through the lenses of cancer biology, stem cell biology, and immunology. Dancer & BioAIMS president.

Honors & Awards

  • Amgen Scholar, Amgen Foundation (Jun - Aug 2014)
  • Gene Brown Prize for Teaching, MIT Department of Biology (June 2015)
  • Stanford Graduate Fellowship, Stanford University
  • NSF Graduate Research Fellowship, National Science Foundation
  • Stanford Biosciences Travel Grant, Stanford Biosciences (Aug 2018)

Professional Affiliations and Activities

  • Member, International Society of Experimental Hematology (2018 - Present)

Membership Organizations

Education & Certifications

  • BS, Massachusetts Institute of Technology, Biology (2015)

Stanford Advisors

Contact

  • amyfan@stanford.edu
    University - Student Department: School of Medicine - IDP's - Immunology Position: Graduate

Additional Info

  • Mail Code: 5421

Links

Current Research and Scholarly Interests

Germline mutations in RUNX1 cause an autosomal dominant disorder characterized by lifelong thrombocytopenia and increased risk of progression to acute myeloid leukemia (AML). Indeed, unlike sporadic AML, which commonly presents in the elderly, the average age of onset for RUNX1 familial AML cases is 35, with over one-third of patients developing leukemia as a child. While megakaryocyte defects have been shown to be a cell-autonomous effect of RUNX1 mutations in hematopoietic stem and progenitor cells (HSPCs), the mechanisms by which germline RUNX1 mutations progress to leukemia remains unclear. Interestingly, RUNX1 is also expressed in bone marrow mesenchymal stromal cells (BM-MSCs), which have been shown to contribute to the pathogenesis of some hematopoietic malignancies. The goal of my thesis research is to determine how RUNX1 mutations may be contributing to leukemogenesis through both cell autonomous and non-autonomous mechanisms.

Lab Affiliations

Work Experience

  • Academic Research Technician, Broad Institute of MIT & Harvard (January 2015 - May 2016)

    Investigate how heterogeneous regulation of LPS modifications by PhoPQ and PmrAB in S. Typhimurium modulate Type I IFN response in mouse macrophages. Developed single-cell RNA-Seq method to simultaneously probe host and pathogen transcriptomes.

    Location

    Cambridge, MA

All Publications

  • Azacitidine and Ascorbate Inhibit the Competitive Outgrowth of Human TET2 Mutant HSPCs in a Xenograft Model of Pre-Leukemia Nakauchi, Y., Thomas, D., Sharma, R., Corces, M., Reinisch, A., Cruz, D., Koehnke, T., Karigane, D., Fan, A., Majeti, R. AMER SOC HEMATOLOGY. 2018

    View details for DOI 10.1182/blood-2018-99-113907

    View details for Web of Science ID 000454837602264

  • scDual-Seq: mapping the gene regulatory program of Salmonella infection by host and pathogen single-cell RNA-sequencing GENOME BIOLOGY Avital, G., Avraham, R., Fan, A., Hashimshony, T., Hung, D. T., Yanai, I. 2017; 18: 200

    Abstract

    The interaction between a pathogen and a host is a highly dynamic process in which both agents activate complex programs. Here, we introduce a single-cell RNA-sequencing method, scDual-Seq, that simultaneously captures both host and pathogen transcriptomes. We use it to study the process of infection of individual mouse macrophages with the intracellular pathogen Salmonella typhimurium. Among the infected macrophages, we find three subpopulations and we show evidence for a linear progression through these subpopulations, supporting a model in which these three states correspond to consecutive stages of infection.

    View details for DOI 10.1186/s13059-017-1340-x

    View details for Web of Science ID 000413769600001

    View details for PubMedID 29073931

    View details for PubMedCentralID PMC5658913

  • A highly multiplexed and sensitive RNA-seq protocol for simultaneous analysis of host and pathogen transcriptomes. Nature protocols Avraham, R., Haseley, N., Fan, A., Bloom-Ackermann, Z., Livny, J., Hung, D. T. 2016; 11 (8): 1477-1491

    Abstract

    The ability to simultaneously characterize the bacterial and host expression programs during infection would facilitate a comprehensive understanding of pathogen-host interactions. Although RNA sequencing (RNA-seq) has greatly advanced our ability to study the transcriptomes of prokaryotes and eukaryotes separately, limitations in existing protocols for the generation and analysis of RNA-seq data have hindered simultaneous profiling of host and bacterial pathogen transcripts from the same sample. Here we provide a detailed protocol for simultaneous analysis of host and bacterial transcripts by RNA-seq. Importantly, this protocol details the steps required for efficient host and bacteria lysis, barcoding of samples, technical advances in sample preparation for low-yield sample inputs and a computational pipeline for analysis of both mammalian and microbial reads from mixed host-pathogen RNA-seq data. Sample preparation takes 3 d from cultured cells to pooled libraries. Data analysis takes an additional day. Compared with previous methods, the protocol detailed here provides a sensitive, facile and generalizable approach that is suitable for large-scale studies and will enable the field to obtain in-depth analysis of host-pathogen interactions in infection models.

    View details for DOI 10.1038/nprot.2016.090

    View details for PubMedID 27442864