Upright Zeiss AxioImager Epifluorescence / Widefield Microscope
The Zeiss AxioImager is a motorized, upright microscope equipped for both transmitted light (DIC, Dark Field, Phase Contrast and Polarization) and fluorescence microscopy. This microscope is equipped for Array Tomography technique, as well as general-purpose widefield fluorescence microscopy.
System specifications:
- Upright Zeiss AxioImager
- Piezo-driven XY scanning stage with linear encoders, travel range:225 - 85mm, maximum speed:100mm/s, resolution:0.2um, reproducibility:<1um, absolute accuracy:+/-5um Motorized Z-drive with 10nm resolution
- DC motorized Z-drive with optoelectronic coding, resolution 10nm, reproducibility 10nm
- EXFO X-Cite 120 illumination source, coupled to scope with liquid light guide, motorized 10-position reflector turret with DIC cube and fluorescence filter cubes (see below)
- Manual 3-position tube lens: mag.1.0X, mag.1.60X
- Bertrand lens 12V/100W halogen light source, manual condenser, 0.9NA, brightfield/DIC/phase contrast/
Detection:
- Axiocam HRm Rev. 3 camera Axiocam 506 Color
Software:
- Zen pro with Deconvolution Module
Objectives (each with DIC slider) |
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mag. |
type |
NA |
||
5X |
EC Epiplan Neofluar |
0.13 |
14.5 |
|
10X |
EC Epiplan Neofluar |
0.25 |
9.0 |
|
20X |
EC Epiplan Neofluar |
0.5 |
2.22 |
|
50X |
EC Epiplan Neofluar |
0.8 |
0.6 |
|
100X (oil) |
EC Epiplan Neofluar |
0.9 |
0.28 |
fluorescence filter sets:(Semrock Brightline #)
|
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description
|
|
dichroic
|
emission
|
|
DAPI (1160A) |
387/11 |
409 |
447/60 |
|
GFP (3035B) |
472/30 |
495 |
520/35 |
|
FITC (3540B) |
482/35 |
506 |
536/40 |
|
YFP (2427A) |
500/24 |
520 |
542/27 |
|
TXRED (4040B) |
562/40 |
593 |
624/40 |
|
CY5 (4040A) |
628/40 |
660 |
692/40 |
Camera Specifications: AxioCam HRm Rev. 3 |
|
Sensor |
Sony ICX 285, progressive readout |
CCD Basic resolution |
1388 X 1040 = 1.4 megapixels |
Pixel size |
6.45um (h) x 6.45um (v) |
Sensor size |
Chip area 8.9 mm x 6.7 mm (equivalent 2/3 inch) |
Dynamic range |
Typical > 1:2200 at 25MHz with <7.7e readout noise???Typical > 1:2500 at 12.5MHz with <6.8e readout noise |
Digitization |
High Quality: 14 bit / 12.5 MHz / High Speed: 12 bit / 25 MHz |
CCD cooling |
Single stage Peltier cooling, regulated |