a, b, EM of the compact myocardium of E10.5 embryos.
c, Quantitative RT-PCR of ventricular α- and β-MHC at E10.5 and E11.5. Ctrl: control. Mut: Sm22αCre;Brg1F/F. p-value: Student's t-test. Error bar: standard deviation.
d, Sequence alignment of the α-MHC locus from mouse, human, and rat. Peak heights indicate degree of sequence homology. Black boxes (a1-a7) are regions of high sequence homology and further analyzed by ChIP. Red: promoter elements. Salmon: introns. Yellow: untranslated regions.
e, PCR of Brg1-immunoprecipitated chromatin from E11.5 hearts. α-HRP: anti-horse radish peroxidase antibody.
f, Luciferase reporter assay of the proximal α-MHC promoter (-462 to +192) in SW13 cells. p-value: Student's t-test. Error bar: standard deviation.
g, Sequence alignment of the β-MHC locus from mouse, human, and rat. Black boxes (b1-b5) are regions of high sequence homology and further analyzed by ChIP. Green: transposons/simple repeats.
h, PCR analysis of Brg1-immunoprecipitated chromatin from E11.5 hearts.
i, Luciferase reporter assays of the β-MHC proximal promoter (-835 to +222) in SW13 cells. p-value: Student's t-test. Error bar: standard deviation.
j, Immunostaining of HDAC1, 2, 3, 5, 6 and 9 (brown) in E11.5 hearts.
k, Co-immunoprecipitation of Brg1 with HDAC1, 2 and 9 in E11.5 hearts.
l, Quantitative RT-PCR of α- and β-MHC of cultured embryos treated with DMSO or TSA. p-value: Student's t-test. Error bar: standard deviation.