The isolated hNSCs were passaged every 5–7 days, as described in the Methods section. They grew as a stable homogenous monolayer and uniformly expressed molecular features of NSCs. The top 3 panels: A, B, C represent photomicrographs of undifferentiated hNSCs immunostained with the neural precursor markers nestin (red, A) and vimentin (green, B) and the radial glial marker 3CB2 (purple, C). The cultures were counterstained with the nuclear DNA marker 4′,6-diamidine-2′-phenylindole dihydrochloride (DAPI, blue). The bottom panels (D, E, F) show 7-day old differentiated hNSC cultures processed for indirect immunocytochemistry. Differentiated hNSCs expressed the neuronal-specific marker TuJ1 (green D, E), the astroglia marker GFAP (red, E), the oligodendroglial marker GC (green, F) and DAPI, blue nuclei in all panels. Cell nuclei were stained with live cell marker DAPI (blue, F, G). Scale bars are 20 µm (A–F).