(A) Schematic overview of the bioinformatics algorithm to compute a three-dimensional model of the otocyst. (B) Transcriptional levels of selected marker genes projected onto 3D model representation. (C) Quantitation of % proportion of cells (left radial pie graph) and fold change between opposing body axes (right radial pie graph) for selected genes as shown in (B). (D) Representative illustration of octant analysis. Corresponding octant number is visualized in red in the 3D otocyst model. Quantitation of expression level and relative number of cells is octant-based. Color-code: d=dorsal (orange), v=ventral (green), l=lateral (blue), m=medial (red), p=posterior (brown), a=anterior (purple). (E) Octant analysis for two candidate genes from the pro-sensory (Sox2 and Lfng) and novel marker category, respectively. See also , and –.