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Technologies Offered:

Light Microscopy

  • Confocal microscopy (scanning and spinning-disk)
  • Two-photon microscopy
  • Wide-field fluorescence microscopy
  • Digital deconvolution
  • Transmitted-light imaging (phase, DIC, histology)
  • Super-resolution imaging (STORM, STED and SIM)
  • Cell surface imaging with <100 nM z-resolution (TIRF)
  • Specialized microscopy (FRAP, FRET, FCS, FLIP...)
  • Fluorescence lifetime imaging microscopy (FLIM)
  • Software for image analysis, processing, and presentation
    • Imaris, Volocity, SVI Huygens, SoftWoRx

Electron Microscopy

Transmission Electron Microscopy (biological)

  • Chemical and cryo-fixation processing
  • immuno-EM
  • Correlative LM to EM: CELM
  • negative staining
  • ultramicrotomy, cryo and plastic
  • electron tomography (plastic sections)

Scanning Electron Microscopy

  • variable pressure/environmental
  • high resolution FE-SEM
  • Immuno-SEM
  • array tomography SEM, CLEM

The Cell Sciences Imaging Facility (CSIF) is a Beckman Center supported, university service center that provides high resolution, state-of-the-art light and electron microscopy technologies for imaging and analyzing the molecular and structural organization of cells, tissue and bioengineered materials.  The CSIF operates two sites at Stanford University: The SOM Beckman Center CSIF and the SOE Shriram Center CSIF.  Both facility sites are open to all members of the Stanford community as well as to external academic and industry researchers (with approval of the facility Director).

 

Events

Leica Light Sheet Microscopy workshop, (starting) September 21st 2015
Monday, September 21, 2015 (All day)

Combining Light Sheet and Confocal Imaging

The Leica TCS SP8 X confocal platform offers advanced features such as the white light laser, light gate, and resonant scanner. Now with DLS (Digital LightSheet) for the Leica TCS SP8, you can have single plane illumination and confocal imaging on one system.

Technical Presentation

September 21th, 12:30-2pm in Room B033 (Shriram Center)

System Demonstrations

September 21st to Oct 15th in Room B023 (Shriram Center)

To reserve a demonstration please go to:

https://sites.google.com/site/leicademos