Pathways of allosteric communication between ATP and the lever arm for the Dictyostelium myosin II pre-stroke conformation. (a) Comparison of shortest paths derived from Identity, WAC, and Miyata matrices for the Dictyostelium pre-stroke structure 1VOM. The number underneath each residue (on a scale from 0 to 9) reflects the degree of relative conservation, with 9 corresponding to the highest conservation. Residues do not necessarily appear with the same conservation level in each row because of different weightings in the three matrices. Residues appearing in all three paths are darkest. (b) Visual representation of Dictyostelium myosin II pre-stroke pathway, computed using the Miyata matrix. The pathway residues (S181, G457, F458, N475, Q479, F482, M486, F487, Q491, Y494, I499, E497, T742) are cyan. The path starts at the ATP γ-phosphate (green). The path ends at T742 of the lever arm (red).