Manu Prakash
Assistant Professor of Bioengineering
Bio
We are a curiosity driven research group working in the field of physical biology. Our approach brings together experimental and theoretical techniques from soft-condensed matter physics, fluid dynamics, theory of computation and unconventional micro and nano-fabrication to open problems in biology: from organismal to cellular and molecular scale. We design and build precision instrumentation including droplet microfluidic tools to probe and perturb biological machines and their synthetic analogues. Along the way, we invent novel technologies in global health context with clinical applications in extreme resource poor settings.
Academic Appointments
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Assistant Professor, Bioengineering
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Member, Bio-X
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Affiliate, Stanford Woods Institute for the Environment
Honors & Awards
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MIT Ideas Sustainability Prize, MIT (2003)
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Lemelson MIT Student Finalist Award, Lemelson Foundation (2008)
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Junior Fellow (Physics), Harvard Society of Fellows (2008-2011)
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TED Senior Fellow, Technology, Entertainment and Design (TED) (2011-2013)
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Frederick E. Terman Fellow, Stanford University (2011-2013)
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Pew Scholar, Pew Foundation (2013-2017)
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TR35, MIT Technology Review (2014)
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Brilliant 10, Popular Science Brilliant 10 (2014)
Professional Education
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Ph.D., Massachusetts Institute of Technology, Field of Study: Applied Physics (MAS) (2008)
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M.S., Massachusetts Institute of Technology, Field of Study: Applied Physics (MAS) (2004)
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B.Tech, Indian Institute of Technology, Field of Study: Computer Science and Engineering (2002)
2015-16 Courses
- Physical Biology
BIOHOPK 320H (Aut) - Physical Biology of Macromolecules
BIOE 41 (Win) -
Independent Studies (5)
- Bioengineering Problems and Experimental Investigation
BIOE 191 (Aut, Win, Spr, Sum) - Directed Investigation
BIOE 392 (Aut, Win, Spr, Sum) - Directed Reading in Biophysics
BIOPHYS 399 (Aut, Win, Spr) - Directed Study
BIOE 391 (Aut, Win, Spr, Sum) - Graduate Research
BIOPHYS 300 (Aut, Win, Spr)
- Bioengineering Problems and Experimental Investigation
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Prior Year Courses
2014-15 Courses
- Organismic Biophysics and Living Soft-matter
BIOE 337 (Win) - Physical Biology of Macromolecules
BIOE 41 (Win)
2013-14 Courses
- Organismic Biophysics and Living Soft-matter
BIOE 337 (Win) - Physical Biology of Macromolecules
BIOE 41 (Win)
2012-13 Courses
- Physical Biology of Macromolecules
BIOE 41 (Win)
- Organismic Biophysics and Living Soft-matter
Stanford Advisees
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Doctoral (Program)
Derek Croote -
Postdoctoral Faculty Sponsor
Shahaf Armon, Felix Jan Hein Hol, Stefan Karpitschka, Vivek Nagendra Prakash -
Postdoctoral Research Mentor
Mohammed Saad Bhamla, Tom Hata, Felix Jan Hein Hol, Stefan Karpitschka
All Publications
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Synchronous universal droplet logic and control
NATURE PHYSICS
2015; 11 (7): 588-596
View details for DOI 10.1038/NPHYS3341
View details for Web of Science ID 000357197300026
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Diagnosis of Schistosoma haematobium Infection with a Mobile Phone-Mounted Foldscope and a Reversed-Lens CellScope in Ghana
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE
2015; 92 (6): 1253-1256
Abstract
We evaluated two novel, portable microscopes and locally acquired, single-ply, paper towels as filter paper for the diagnosis of Schistosoma haematobium infection. The mobile phone-mounted Foldscope and reversed-lens CellScope had sensitivities of 55.9% and 67.6%, and specificities of 93.3% and 100.0%, respectively, compared with conventional light microscopy for diagnosing S. haematobium infection. With conventional light microscopy, urine filtration using single-ply paper towels as filter paper showed a sensitivity of 67.6% and specificity of 80.0% compared with centrifugation for the diagnosis of S. haematobium infection. With future improvements to diagnostic sensitivity, newer generation handheld and mobile phone microscopes may be valuable tools for global health applications.
View details for DOI 10.4269/ajtmh.14-0741
View details for Web of Science ID 000355785400028
View details for PubMedID 25918211
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Vapour-mediated sensing and motility in two-component droplets.
Nature
2015; 519 (7544): 446-450
Abstract
Controlling the wetting behaviour of liquids on surfaces is important for a variety of industrial applications such as water-repellent coatings and lubrication. Liquid behaviour on a surface can range from complete spreading, as in the 'tears of wine' effect, to minimal wetting as observed on a superhydrophobic lotus leaf. Controlling droplet movement is important in microfluidic liquid handling, on self-cleaning surfaces and in heat transfer. Droplet motion can be achieved by gradients of surface energy. However, existing techniques require either a large gradient or a carefully prepared surface to overcome the effects of contact line pinning, which usually limit droplet motion. Here we show that two-component droplets of well-chosen miscible liquids such as propylene glycol and water deposited on clean glass are not subject to pinning and cause the motion of neighbouring droplets over a distance. Unlike the canonical predictions for these liquids on a high-energy surface, these droplets do not spread completely but exhibit an apparent contact angle. We demonstrate experimentally and analytically that these droplets are stabilized by evaporation-induced surface tension gradients and that they move in response to the vapour emitted by neighbouring droplets. Our fundamental understanding of this robust system enabled us to construct a wide variety of autonomous fluidic machines out of everyday materials.
View details for DOI 10.1038/nature14272
View details for PubMedID 25762146
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Vapour-mediated sensing and motility in two-component droplets
NATURE
2015; 519 (7544): 446-?
View details for DOI 10.1038/nature14272
View details for Web of Science ID 000351602800051
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Punch Card Programmable Microfluidics
PLOS ONE
2015; 10 (3)
View details for DOI 10.1371/journal.pone.0115993
View details for Web of Science ID 000350685900006
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Punch card programmable microfluidics.
PloS one
2015; 10 (3)
Abstract
Small volume fluid handling in single and multiphase microfluidics provides a promising strategy for efficient bio-chemical assays, low-cost point-of-care diagnostics and new approaches to scientific discoveries. However multiple barriers exist towards low-cost field deployment of programmable microfluidics. Incorporating multiple pumps, mixers and discrete valve based control of nanoliter fluids and droplets in an integrated, programmable manner without additional required external components has remained elusive. Combining the idea of punch card programming with arbitrary fluid control, here we describe a self-contained, hand-crank powered, multiplex and robust programmable microfluidic platform. A paper tape encodes information as a series of punched holes. A mechanical reader/actuator reads these paper tapes and correspondingly executes operations onto a microfluidic chip coupled to the platform in a plug-and-play fashion. Enabled by the complexity of codes that can be represented by a series of holes in punched paper tapes, we demonstrate independent control of 15 on-chip pumps with enhanced mixing, normally-closed valves and a novel on-demand impact-based droplet generator. We demonstrate robustness of operation by encoding a string of characters representing the word "PUNCHCARD MICROFLUIDICS" using the droplet generator. Multiplexing is demonstrated by implementing an example colorimetric water quality assays for pH, ammonia, nitrite and nitrate content in different water samples. With its portable and robust design, low cost and ease-of-use, we envision punch card programmable microfluidics will bring complex control of microfluidic chips into field-based applications in low-resource settings and in the hands of children around the world.
View details for DOI 10.1371/journal.pone.0115993
View details for PubMedID 25738834
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Emergent mechanics of biological structures
MOLECULAR BIOLOGY OF THE CELL
2014; 25 (22): 3461-3465
View details for DOI 10.1091/mbc.E14-03-0784
View details for Web of Science ID 000344236800007
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Emergent mechanics of biological structures.
Molecular biology of the cell
2014; 25 (22): 3461-3465
Abstract
Mechanical force organizes life at all scales, from molecules to cells and tissues. Although we have made remarkable progress unraveling the mechanics of life's individual building blocks, our understanding of how they give rise to the mechanics of larger-scale biological structures is still poor. Unlike the engineered macroscopic structures that we commonly build, biological structures are dynamic and self-organize: they sculpt themselves and change their own architecture, and they have structural building blocks that generate force and constantly come on and off. A description of such structures defies current traditional mechanical frameworks. It requires approaches that account for active force-generating parts and for the formation of spatial and temporal patterns utilizing a diverse array of building blocks. In this Perspective, we term this framework "emergent mechanics." Through examples at molecular, cellular, and tissue scales, we highlight challenges and opportunities in quantitatively understanding the emergent mechanics of biological structures and the need for new conceptual frameworks and experimental tools on the way ahead.
View details for DOI 10.1091/mbc.E14-03-0784
View details for PubMedID 25368421
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Foldscope: Origami-Based Paper Microscope
PLOS ONE
2014; 9 (6)
View details for DOI 10.1371/journal.pone.0098781
View details for Web of Science ID 000338508200010
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Probing the Mechanical Coupling of the Cell Membrane to the Nucleus with Vertical Nanopillar Arrays
CELL PRESS. 2013: 546A-546A
View details for Web of Science ID 000316074305286
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Hydraulic stress induced bubble nucleation and growth during pupal metamorphosis
OXFORD UNIV PRESS INC. 2012: E140-E140
View details for Web of Science ID 000303165001028
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Flying in two dimensions
OXFORD UNIV PRESS INC. 2012: E141-E141
View details for Web of Science ID 000303165001029
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The hungry fly: Hydrodynamics of feeding in the common house fly
PHYSICS OF FLUIDS
2011; 23 (9)
View details for DOI 10.1063/1.3640023
View details for Web of Science ID 000295621800010
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Face-selective electrostatic control of hydrothermal zinc oxide nanowire synthesis
NATURE MATERIALS
2011; 10 (8): 596-601
Abstract
Rational control over the morphology and the functional properties of inorganic nanostructures has been a long-standing goal in the development of bottom-up device fabrication processes. We report that the geometry of hydrothermally grown zinc oxide nanowires can be tuned from platelets to needles, covering more than three orders of magnitude in aspect ratio (~0.1-100). We introduce a classical thermodynamics-based model to explain the underlying growth inhibition mechanism by means of the competitive and face-selective electrostatic adsorption of non-zinc complex ions at alkaline conditions. The performance of these nanowires rivals that of vapour-phase-grown nanostructures, and their low-temperature synthesis (<60 °C) is favourable to the integration and in situ fabrication of complex and polymer-supported devices. We illustrate this capability by fabricating an all-inorganic light-emitting diode in a polymeric microfluidic manifold. Our findings indicate that electrostatic interactions in aqueous crystal growth may be systematically manipulated to synthesize nanostructures and devices with enhanced structural control.
View details for DOI 10.1038/NMAT3069
View details for Web of Science ID 000293000000019
View details for PubMedID 21743451
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Hydraulic stress induced bubble nucleation and growth during pupal metamorphosis
AMER SOC CELL BIOLOGY. 2011
View details for Web of Science ID 000305505501236
- Face-selective electrostatic control of nanowire synthesis Nature Materials 2011; 10: 596-601
- Interfacial Propulsion by Directional Adhesion International Journal of Non-Linear Mechanics 2011; 46 (4): 607-615
- On a tweezer for droplets Advances in Colloid and Interface Science 2010; 161: 10-14
- Drop propulsion in tapered tubes Euro Physics Letters, 2009; 86: 1-5
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Surface tension transport of prey by feeding shorebirds: The capillary ratchet
SCIENCE
2008; 320 (5878): 931-934
Abstract
The variability of bird beak morphology reflects diverse foraging strategies. One such feeding mechanism in shorebirds involves surface tension-induced transport of prey in millimetric droplets: By repeatedly opening and closing its beak in a tweezering motion, the bird moves the drop from the tip of its beak to its mouth in a stepwise ratcheting fashion. We have analyzed the subtle physical mechanism responsible for drop transport and demonstrated experimentally that the beak geometry and the dynamics of tweezering may be tuned to optimize transport efficiency. We also highlight the critical dependence of the capillary ratchet on the beak's wetting properties, thus making clear the vulnerability of capillary feeders to surface pollutants.
View details for DOI 10.1126/science.1156023
View details for Web of Science ID 000255868300042
View details for PubMedID 18487193
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Microfluidic bubble logic
SCIENCE
2007; 315 (5813): 832-835
Abstract
We demonstrate universal computation in an all-fluidic two-phase microfluidic system. Nonlinearity is introduced into an otherwise linear, reversible, low-Reynolds number flow via bubble-to-bubble hydrodynamic interactions. A bubble traveling in a channel represents a bit, providing us with the capability to simultaneously transport materials and perform logical control operations. We demonstrate bubble logic AND/OR/NOT gates, a toggle flip-flop, a ripple counter, timing restoration, a ring oscillator, and an electro-bubble modulator. These show the nonlinearity, gain, bistability, synchronization, cascadability, feedback, and programmability required for scalable universal computation. With increasing complexity in large-scale microfluidic processors, bubble logic provides an on-chip process control mechanism integrating chemistry and computation.
View details for DOI 10.1126/science.1136907
View details for Web of Science ID 000244069000065
View details for PubMedID 17289994
- The Integument of Water-walking Arthropods: Form and Function Advances in Insect Physiology 2007; 34: 117-192
- Water walking devices Experiments in Fluids 2007; 43: 769-778
- Microfludic Bubble Logic Science 2007; 315: 832-835
- Personal fabrication Telektronikk 2004; 3: 22-26