A complex network of factors orchestrates cell division, including those that regulate DNA synthesis and mitosis. Mitosis is particularly dynamic as chromosomes are repositioned into different cells with the assistance of the mitotic spindle, the accuracy of which is critical to faithful transmission of genetic material. Spindle positioning relies on the function of molecular motors, such as kinesins (plus-end directed) and dyneins (minus-end directed), to generate ‘push and pull’ forces to orient the spindle and separate sister chromatids to opposite poles of the cells. Specifically, kinesin-related microtubule motors are involved in microtubule movement spindle positioning, including attachment to cell cortex. Microtubule-associated proteins also facilitate attachments between microtubule and cell cortex/ kinetochore, respectively.
Alterations in nucleosome composition and positioning throughout the genome influence structural changes in chromatin, which subsequently contribute to proper chromosome segregation. Using a varied approach including genetic, molecular, and cellular techniques (Figure 1), we are investigating the relationship between chromatin-remodeling complexes that affect nucleosome function and chromosome segregation.
FIG 1. Live imaging of S.cerevisiae expressing H2B-GFP in CellAsic microfluidic chamber.