Format

Send to

Choose Destination
Tissue Eng. 2007 Jun;13(6):1159-71.

Hypoxia inducible factor-1alpha deficiency affects chondrogenesis of adipose-derived adult stromal cells.

Author information

1
Children's Surgical Research Program, Department of Surgery, Stanford University School of Medicine, Stanford, California 94305, USA.

Abstract

Increased cartilage-related disease, poor regeneration of cartilage tissue, and limited treatment options have led to intense research in tissue engineering of cartilage. Adipose-derived adult stromal cells (ADAS) are a promising cell source for skeletal tissue engineering; understanding ADAS cellular signaling and chondrogenesis will advance cell-based therapies in cartilage repair. Chondrocytes are unique-they are continuously challenged by a hypoxic microenvironment. Hypoxia inducible factor-1-alpha (HIF-1alpha), a critical mediator of a cell's response to hypoxia, plays a significant role in chondrocyte survival, growth arrest, and differentiation. By using an established in vitro 3-dimensional micromass system, we investigated the role of HIF-1alpha in chondrogenesis. Targeted deletion of HIF-1alpha in ADAS substantially inhibited the chondrogenic pathway specifically. In marked contrast, deletion of HIF-1alpha did not affect osteogenic differentiation but enhanced adipogenic differentiation. This study demonstrates the critical and specific interplay between HIF-1alpha and chondrogenesis in vitro.

PMID:
17518738
DOI:
10.1089/ten.2006.0265
[Indexed for MEDLINE]

Supplemental Content

Loading ...
Support Center