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A high-resolution map of transcription in the yeast genome
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Contributed by Ronald W. Davis, February 10, 2006
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↵ †L.D. and W.H. contributed equally to this work.
Abstract
There is abundant transcription from eukaryotic genomes unaccounted for by protein coding genes. A high-resolution genome-wide survey of transcription in a well annotated genome will help relate transcriptional complexity to function. By quantifying RNA expression on both strands of the complete genome of Saccharomyces cerevisiae using a high-density oligonucleotide tiling array, this study identifies the boundary, structure, and level of coding and noncoding transcripts. A total of 85% of the genome is expressed in rich media. Apart from expected transcripts, we found operon-like transcripts, transcripts from neighboring genes not separated by intergenic regions, and genes with complex transcriptional architecture where different parts of the same gene are expressed at different levels. We mapped the positions of 3′ and 5′ UTRs of coding genes and identified hundreds of RNA transcripts distinct from annotated genes. These nonannotated transcripts, on average, have lower sequence conservation and lower rates of deletion phenotype than protein coding genes. Many other transcripts overlap known genes in antisense orientation, and for these pairs global correlations were discovered: UTR lengths correlated with gene function, localization, and requirements for regulation; antisense transcripts overlapped 3’ UTRs more than 5’ UTRs; UTRs with overlapping antisense tended to be longer; and the presence of antisense associated with gene function. These findings may suggest a regulatory role of antisense transcription in S. cerevisiae. Moreover, the data show that even this well studied genome has transcriptional complexity far beyond current annotation.
Proteins constitute most structural and functional components of cells. The assumption has been that protein-encoding genes are also the main controllers of cellular processes. Recent evidence challenges this assumption, suggesting a wide-spread involvement of noncoding RNA in regulation, including through the activity of untranslated regions of mRNAs (1), antisense transcripts (2, 3), and isolated noncoding RNAs such as microRNA that control transcript levels or their translation (4).
High-resolution transcriptome analysis in higher eukaryotes using tiling arrays has improved ORF annotations and exon-intron predictions and discovered many new transcripts of currently unknown function (5–7). However, these studies have encountered challenges, due to noise, limited resolution, lack of strand-specific signal, and drawbacks in the analysis methods (8). Sequencing of cloned cDNAs has also revealed a high level of transcriptional complexity, including the presence of many new transcripts, alternative promoter usage, splicing, and polyadenylation, as well as the presence of many sense–antisense transcript pairs (3, 9). However, because of the cost and labor of large-scale sequencing, this approach has been limited. Therefore, there is a need to develop high-throughput, precise, and high-resolution technology to map the full transcriptional activity. Yeast is a simple and relatively small eukaryotic genome that provides opportunities to rapidly characterize novel findings.
We developed an oligonucleotide array for Saccharomyces cerevisiae that contains 6.5 million probes and interrogates both strands of the full genomic sequence with 25-mer probes tiled at an average of eight nucleotide intervals on each strand (17 nucleotides overlap) and a four nucleotides offset of the tile between strands. This design enables a 4-nt resolution for hybridization of double stranded targets and an 8-nt resolution for strand-specific targets. We profiled transcription during exponential growth in rich media, the standard laboratory growth condition, to generate a comprehensive map of transcription.
Results and Discussion
Microarray Experiments and Analysis.
We hybridized first-strand cDNA synthesized using random primers from polyadenylated [poly(A)] and total RNA. To calibrate the sequence-specific probe effect (10–12), we background-corrected and adjusted (13) the signal of each probe by sequence-specific parameters, estimated from a calibration set of genomic DNA hybridizations (Fig. 5, which is published as supporting information on the PNAS web site). This method allowed us to quantitatively compare the signal from probe to probe on the array.
The Transcriptome.
To address the question of how much of the genome is transcribed, we analyzed the coding regions of 5,654 ORFs that were annotated as verified or uncharacterized genes in the Saccharomyces Genome Database (SGD, www.yeastgenome.org) and represented by unique probes on the array. Significant expression above background was detected for 5,104 ORFs (90%) (Binomial test, false discovery rate = 0.001; Fig. 6, which is published as supporting information on the PNAS web site). As expected, genes that were not detected have functions not required in this condition such as meiosis, sporulation, mating, sugar transport, and vitamin metabolism [hypergeometric test for gene ontology (GO) annotation enrichment, unadjusted P ≤ 3 × 10−9]. In addition, analyzing 11,412,997 bp of unique genomic sequence, we detected expression above background on either strand for 85%. Comparing this to existing annotation, which covers ≈75% of the genome, shows that 16% of the transcribed base pairs had not been annotated before.
To obtain an unbiased map of the position, abundance, and architecture of transcripts, the hybridization signals were examined along their chromosomal position for each strand (Fig. 1). The profiles were partitioned into segments of constant hybridization intensity, separated by change points demarcating transcript boundaries. We used a change point detection algorithm that determines the global maximum of the log-likelihood of a piece-wise constant model by dynamic programming (14, 15). Compared to running-window approaches, it finds more accurate estimates of change point locations and depends on fewer user-defined parameters. Segments were determined separately for poly(A) and total RNA (Tables 3 and 4, respectively, which are published as supporting information on the PNAS web site). Segments from poly(A) and total RNA were remarkably concordant, and many noncoding RNA (ncRNA) were also found in the poly(A) data (Table 5, which is published as supporting information on the PNAS web site). Overall, the poly(A) RNA hybridization data were cleaner and therefore were the focus of our analysis.
The automated segmentation algorithm provides an unbiased global analysis, but the data complexity invites additional manual curation. Profiles for all genomic regions are provided in a database that is searchable by gene symbol or chromosomal coordinate (www.ebi.ac.uk/huber-srv/queryGene). We encourage readers to explore the database along with the examples discussed below.
Examples from this map of transcription are shown in Fig. 2. The hybridization data accurately separates exons from spliced introns, for which lower but significant levels of transcription were found, as shown for RPS16A (top strand) and RPL13B (bottom strand) (Fig. 2 a). The segmentation-mapped UTRs of coding transcripts (e.g. GCN4, Fig. 2 b) identified complex transcriptional architectures, such as uneven transcript levels for different regions of a single ORF (MET7, Fig. 2 c), determined transcripts spanning multiple ORFs (YCK2, GIM3, Fig. 2 d), and identified adjacent transcripts for neighboring genes, uninterrupted by untranscribed intergenic regions (SRG1, SER3, Fig. 2 e). Moreover, we observed transcripts in regions of the genome lacking prior annotation (Fig. 2 f), as well as transcripts opposite annotated features (Fig. 2 g). For each of the above, many instances were identified in the genome and we discuss them below.
UTR Boundaries.
To map UTRs, we compared ORF boundaries with segment boundaries. We automatically determined UTR lengths for verified or uncharacterized nuclear-encoded genes whose annotated coding sequence was fully contained within a single segment. A total of 2,223 segments passed a confidence filter that required a sharp decrease in signal on both sides of the segment. UTR coordinates are given in Tables 3 and 4. We proceeded with analysis of the 2,044 poly(A)-determined UTRs because the poly(A) hybridization data were cleaner and yielded most of the UTR determinations (Fig. 7, which is published as supporting information on the PNAS web site). For many remaining genes that did not pass the confidence filter, the UTRs can be mapped by closer inspection.
We found that 3′ UTRs were significantly longer than the 5′ UTRs, with a median of 91 vs. 68 nt (Fig. 3 a). Longer 3′ UTRs are consistent with them containing posttranscriptional regulatory regions that influence mRNA stability, localization, and translation (16), and with findings from other species (17). The mean sum of 3′ and 5′ UTR lengths was 211 nt and similar to a mean of 256 nt found by a gel-mobility assay (18). We computed 662 3′ UTRs from ESTs (19) and compared them to 435 ORFs that had UTRs in our data set. The Pearson correlation coefficient between the UTR length estimates was 0.63. A contribution to the differences is that in the EST data the longest transcript was chosen, whereas the array measures the average transcript abundance at each probe position.
We compared UTR lengths with transcript levels and coding sequence (ORF) lengths. Although transcript level was generally lower for genes with long coding sequences, neither transcript levels nor ORF lengths were significantly associated with UTR lengths. We also compared length distributions of UTRs for different functional and localization categories (GO annotations) and detected significant correlations (Fig. 3 b). The longest 3′ UTRs were found for transcripts of proteins that are targeted to the mitochondrial electron transport chain, the plasma membrane, and the cell wall. These longer 3′ UTRs may contain mRNA localization signals, as has been well demonstrated for mitochondrial targeted proteins (20, 21). Genes involved in phosphorylation, transporter activity, ion transport, and specific stages of the mitotic cell cycle had both ends longer. Genes involved in RNA processing, rRNA metabolism, and ribosome biogenesis had both ends shorter. Therefore, genes with longer UTRs seem to fall into categories that require regulation, whereas genes with short UTRs seem to fall into categories with a reduced need for posttranscriptional regulation, such as housekeeping genes.
Complex Transcriptional Architectures.
Many expressed segments flanked other expressed segments with different signal levels, thus making up complex transcriptional architectures. In many cases, different parts of the same gene are expressed at different levels: 921 ORFs from the poly(A) RNA sample were divided into at least two expressed segments, one covering >50% of the feature and others <50%. Such complex architectures could be due to alternative transcription initiation, termination, or alternative splicing, as has been described in mouse (9) and for several human genes (22). In yeast, it has been suggested that up to 20% of mRNAs have alternative 3′ ends (23). Complex hybridization patterns on the array could also be caused by RNA decay or variation introduced by reverse transcription, because the array captures the sum of cDNA molecules present at the time of hybridization. The explanation of our observations by such mechanisms will require a case-by-case analysis.
Here, we discuss a few cases. For CPB1 and RNA14, our observed architecture matches previous results describing alternative 3′ ends in response to carbon source regulation (24). For GCN4, lower hybridization signal was observed at the 3′ end (Fig. 2 b). GCN4 is not translated during nutrient-rich growth because of the translation of the upstream ORFs encoded in the same transcript (25). 3′ end degradation due to a lack of translation could explain the lower 3′ end signal. In support, this decrease was not seen in an oligo(dT) reverse-transcribed sample, where no priming would occur on degraded poly(A) transcripts. At the 5′ end, the segment boundary matches the previously determined position to within nine bases. For MET7, the annotated gene was segmented into three regions (Fig. 2 c), suggesting a misannotation of the translation start site. A later transcription start site is supported by the multiple sequence alignment of yeast species in SGD, which shows that conservation of MET7 starts at a later methionine (M55), whose position agrees with the transcription start site detected by the array. Also, the level difference between the central and the 3′ segment was not seen in a poly(A) sample that was reverse transcribed by using oligo(dT) primers (Fig. 8, which is published as supporting information on the PNAS web site), consistent with early transcript termination or RNA decay. Altogether, we tested 27 regions from 10 genes by quantitative real-time PCR (including MET7). For seven genes, the PCR results matched the architectures in the array data (Fig. 8).
Neighboring Transcription.
Additional unusual architecture was found for adjacent ORFs not separated by an unexpressed region. Such architectures can result from more than one ORF being encoded from a single transcript, like the upstream ORFs in GCN4, or from distinct transcripts not separated by untranscribed intergenic regions. We found the ORFs of GIM3 and YCK2 within one segment resembling a bicistronic transcript (Fig. 2 d). The PhastCons multiple alignment (26) of the intergenic region with other yeast species shows high sequence conservation, but includes frame-shifting gaps, which suggests that the two ORFs are not translated as one. By reverse-transcription PCR across the gap between the ORFs, a product was obtained supporting either bicistronic or overlapping transcripts. Operon-like transcription was reported for few eukaryotic species and mostly for Caenorhabditis elegans (27). A bicistronic transcript had been reported previously in yeast for YMR181C and RGM1 (28), and we observed different transcript levels for the ORFs, but no separation by an untranscribed region.
Fig. 2 e shows two other adjacent transcripts, SRG1 and SER3, expressed at different levels and not separated by an intergenic region. It had been proposed that SRG1, an upstream noncoding RNA, represses the expression of SER3 in rich media, by reducing the binding of SER3 transcription factors (29). In contrast, we find that SER3 is expressed significantly above background, suggesting that even though SRG1 is expressed at a higher level, its transcription does not prevent SER3 from being transcribed. There are many cases of adjacent genes not separated by unexpressed, intergenic regions in our data set, and this suggests that transcription over active promoters of adjacent genes is common in yeast. Some further examples are QCR6, PHO8, RIB3, HCH1, UBI4, SEC53, RPS26A, and ADE13.
Unannotated Transcripts.
Many segments with signal above background did not overlap existing annotation. They fall into two classes: nonannotated isolated segments if there was no prior annotation on either strand (Fig. 2 f), and nonannotated antisense segments if there was an annotation on the opposite strand (Fig. 2 g). Many are not independent transcription units: some represent UTRs of genes with complex transcriptional architecture; others are part of unannotated transcripts that are divided into multiple segments. The identification of antisense transcripts requires caution because reverse transcription can generate double stranded cDNA from secondary mispriming (30, 31). Considering these concerns, we applied a filter requiring segments to be at least 48 bp long, be flanked by segments with reduced hybridization signal on both sides, and have higher expression signal than seen on the opposite strand for at least part of their length. In these filtered categories, we obtained 427 nonannotated segments from poly(A) and 357 from total RNA hybridizations. These segments divide approximately equally into the isolated and antisense categories (Fig. 4 a). Antisense segments and segments overlapping annotation (≥50%) had similar length distributions and tended to be longer than those of the isolated categories (Fig. 4 b). Isolated segments showed similar levels of expression as annotated segments, whereas antisense segments had lower expression levels (Fig. 4 c).
Nonannotated Isolated Transcripts.
We verified the array identification of 126 nonannotated isolated transcripts by RT-PCR. All were expressed in both total and poly(A) RNA reverse transcribed by using random and oligo(dT) primers, respectively. For 10 of them, a quantitative real-time PCR analysis showed their levels to be similar to expressed ORFs in both sample types.
The 1.7-kb transcript between ORC2 and TRM7 (Fig. 2 f) is an example of a nonannotated isolated segment that is highly conserved across other yeast species in the PhastCons multiple alignment (26). Nevertheless, assessment of 125 nonannotated isolated segments showed that only 48 had a multiple alignment >50 nt across four yeast species (32). In addition, median total tree lengths of the phylogenies were 0.59 for verified genes, 1.08 for undetected unannotated segments, and 1.50 for nonannotated isolated segments (Table 6, which is published as supporting information on the PNAS web site). To assess protein-coding potential, we tested for dissimilarity in evolutionary rates among first, second, and third codon positions in all reading frames. There was no protein coding signature for the 48 nonannotated segments (median likelihood-ratio statistic of 1.06, compared to 1.14 for undetected unannotated segments and 162.0 for verified genes). Conservation of DNA sequence or protein coding ability is nevertheless neither a necessary nor a sufficient attribute of transcript function.
We generated knockouts for 47 nonannotated isolated segments and tested for growth defects in rich media conditions (Table 7, which is published as supporting information on the PNAS web site). A growth defect was identified for two knockouts: one on chromosome 6, positions 54813–55221, the other on chromosome 7, positions 622039–622295. On chromosome 6, the deleted segment contained annotated transcription factor binding sites upstream to ACT1, an essential gene, which likely accounts for the observed inviability. On chromosome 7, the deletion does not overlap any annotation, and strains with deletions of the neighboring ORFs (YGR066C, YGR067C) did not have a growth defect. This segment does not appear to be evolutionarily conserved or to contain a long ORF. The proportion of growth defects found within the 47 knockouts is much lower than the ≈40% found for knockouts of protein-coding genes (33).
Nonannotated Antisense Transcription.
We identified antisense transcripts opposite to 1,555 genes, of which 402 were in the filtered set from both poly(A) and total RNA samples (Tables 3 and 4). The antisense transcripts are not caused by read-through from ORFs on the opposite strand, but appear as independent transcription units. For example, antisense transcription was found opposite SPO22, a meiosis-specific protein induced early in meiosis (Fig. 2 g). Upstream of this antisense transcript, there is a binding site for CBF1. CBF1 is involved in regulation of DNA replication and chromosome cycle and is important for growth in rich media, suggesting that the antisense expression may be negatively correlated with the expression of SPO22.
Many genes with antisense transcripts had products that localize to the cell cortex and cell wall, and that function in the meiotic cell cycle and in transcriptional regulation (Table 1). Some of these categories included genes not active during growth in rich media, like meiosis. Others included genes that are active during growth in rich media, but which may need posttranscriptional regulation. Further correlations were found between UTRs and their opposite antisense segments: More antisense transcripts overlapped the 3′ UTRs than the 5′ UTRs; also, UTRs that had overlapping antisense transcripts were longer than UTRs that did not (Table 2).
The generation and significance of the many nonannotated transcripts is unclear. Regulation of gene expression by antisense transcripts was reported in prokaryotes (34) and higher eukaryotes (35). Sense/antisense transcript pairs were suggested to be frequent in mammalian genomes and to provide regulatory function (3). In S. cerevisiae, major components of the RNA interference machinery have not been identified (36); however, in other species, alternative mechanisms for regulation by noncoding RNAs exist (2, 37). In Drosophila, it has been shown that microRNA predominantly target 3′ UTRs (38) and these UTRs also tend to be longer than UTRs of genes not targeted (39). We observed similar correlations for antisense transcripts in S. cerevisiae, which together with their association to particular functional categories may suggest a possible regulatory role. There are experiments supporting this hypothesis: artificial antisense transcripts in S. cerevisiae had effects on expression of several genes (40–42), and overexpression of random genomic fragments antisense to ORFs has led in several cases to growth inhibition (43). In our data set, naturally occurring antisense transcripts were found for ≈20 of these cases.
Most ncRNAs previously reported as novel have since been annotated in SGD, and hence do not overlap with our expressed, nonannotated segments (44, 45). We compared our data to transcriptome surveys, carried out by using serial analysis of gene expression (SAGE) (46) and ESTs (19). Thirteen percent of the nonannotated isolated and 42% of the nonannotated antisense transcripts were represented by SAGE tags. For the EST data, these numbers were 1% and 6%, respectively. Analysis of SAGE tags on microarrays described a number of novel transcripts in a mutant strain defective in the RNA degradation pathway (47); however, the eight primary examples were not found expressed in our study of wild-type yeast.
This study reveals considerable transcriptional activity in yeast that is currently not systematically annotated. Our transcription map will be useful for annotating the genome. Furthermore, the position of transcription initiation and termination sites will help in defining the promoters and transcriptional regulators of genes. Although our results suggest that not many new, long protein-coding regions will be discovered in yeast, the extensive noncoding transcription detected in regions with no prior anotation and antisense to annotated transcripts invites further investigation. Therefore, even for a genome that has been studied intensively since it was sequenced 10 years ago (48), a glimpse into the complexity of its transcriptional architecture makes this genome appear like novel territory.
Materials and Methods
Array Design and Sample Hybridization.
The array was designed in collaboration with Affymetrix (Santa Clara, CA) (PN 520055). An S288c background strain S96 (MATa gal2 lys5) was grown in rich yeast-extract/peptone/dextrose media to mid-exponential phase. Total RNA was isolated by hot phenol extraction. Poly(A) RNA was enriched by two rounds of the Oligotex mRNA kit (Qiagen). First-strand cDNA was synthesized by using random primers. Three replicate hybridizations (biological) of poly(A), two of total RNA, and three of genomic DNA were performed.
Probe Annotation.
Probe sequences were aligned to the genome sequence of S. cerevisiae strain S288c (SGD of August 7, 2005). Perfect match probes were further analyzed.
Normalization.
RNA hybridization intensities were adjusted by where X ij is the RNA intensity of the ith probe on the jth array, A i is the geometric mean of the intensities from the DNA hybridizations, B j (A) is a continuous function that parameterizes the estimated background of probes with gain A, and N ij is the adjusted intensity. Probes were grouped into 20 strata defined by the 5%, 10%, 15%, …, 100% quantiles of A i . Within each stratum, and for each array j, the midpoint of the shorth of the intensities of the probes for which no genomic feature was annotated on either strand was calculated. Linear interpolation yielded the function B j . Dead probes (the 5% of probes with lowest signal in the DNA hybridization) were discarded. The values N ij were background-adjusted and transformed to log 2 scale by using vsn (13). Fig. 5 demonstrates the successive improvements in the signal-to-noise ratio during normalization.
Segmentation.
Segments of approximately constant hybridization signal were defined by using a dynamic programming algorithm that, for each chromosome strand separately, minimizes the cost function where yij is the VSN-normalized signal of the ith probe on the jth replicate array, ȳsj is the arithmetic mean of the signal values of array j in segment s, S is the number of segments, and t1 , …, t S are the segment boundaries (15). For each chromosome, S was chosen such that the average segment length was 1,500 nt. S, the only parameter of the segmentation algorithm, controls the sensitivity–specificity tradeoff and was chosen to yield high sensitivity.
All analyses were performed with custom-written software in the language and statistics environment r (49) and bioconductor (14). For additional details on analyses and experimental procedure, see Supporting Text, which is published as supporting information on the PNAS web site.
Acknowledgments
We thank Mike Mittmann for the array design, Iain Russell and Victor Sementchenko for experimental advice, Raquel Kuehn and Michelle Nugyen for technical assistance, Roy Parker, Rafael Irizarry, Elisa Izaurralde, Steve Cohen, and Nick Goldman for helpful comments on the manuscript, and contributors to the bioconductor (www.bioconductor.org) and r (www.R-project.org) projects for their software. This work was funded by the National Institutes of Health (R.W.D and L.M.S.) and the Deutsche Forschungsgemeinschaft (L.M.S.).
Footnotes
- ¶To whom correspondence may be addressed. E-mail: dbowe{at}stanford.edu or larsms{at}embl.de
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Author contributions: W.H., R.W.D., and L.M.S. designed research; L.D., W.H., M.G., and L.M.S. performed research; C.J.P. and T.J. contributed new reagents/analytic tools; L.D., W.H., M.G., J.T., L.B., and L.M.S. analyzed data; and L.D., W.H., and L.M.S. wrote the paper.
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Conflict of interest statement: No conflicts declared.
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Data deposition: The array data have been deposited in ArrayExpress database (accession no. E-TABM-14).
- Abbreviations:
- GO,
- gene ontology.
Abbreviation:
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Freely available online through the PNAS open access option.
- © 2006 by The National Academy of Sciences of the USA
References
- ↵
- ↵
-
↵
- Katayama S. ,
- Tomaru Y. ,
- Kasukawa T. ,
- Waki K. ,
- Nakanishi M. ,
- Nakamura M. ,
- Nishida H. ,
- Yap C. C. ,
- Suzuki M. ,
- Kawai J. ,
- et al.
- ↵
-
↵
- Cheng J. ,
- Kapranov P. ,
- Drenkow J. ,
- Dike S. ,
- Brubaker S. ,
- Patel S. ,
- Long J. ,
- Stern D. ,
- Tammana H. ,
- Helt G. ,
- et al.
-
↵
- Bertone P. ,
- Stolc V. ,
- Royce T. E. ,
- Rozowsky J. S. ,
- Urban A. E. ,
- Zhu X. ,
- Rinn J. L. ,
- Tongprasit W. ,
- Samanta M. ,
- Weissman S. ,
- et al.
-
↵
- Yamada K. ,
- Lim J. ,
- Dale J. M. ,
- Chen H. ,
- Shinn P. ,
- Palm C. J. ,
- Southwick A. M. ,
- Wu H. C. ,
- Kim C. ,
- Nguyen M. ,
- et al.
- ↵
-
↵
- Carninci P. ,
- Kasukawa T. ,
- Katayama S. ,
- Gough J. ,
- Frith M. C. ,
- Maeda N. ,
- Oyama R. ,
- Ravasi T. ,
- Lenhard B. ,
- Wells C. ,
- et al.
-
↵
- Hekstra D. ,
- Taussig A. R. ,
- Magnasco M. ,
- Naef F.
- ↵
- ↵
- ↵
-
↵
- Gentleman R. ,
- Carey V. ,
- Huber W. ,
- Irizarry R. ,
- Dudoit S.
- ↵
- ↵
- ↵
- ↵
-
↵
- Graber J. H. ,
- Cantor C. R. ,
- Mohr S. C. ,
- Smith T. F.
- ↵
- ↵
-
↵
- Kapranov P. ,
- Drenkow J. ,
- Cheng J. ,
- Long J. ,
- Helt G. ,
- Dike S. ,
- Gingeras T. R.
-
↵
- Graber J. H. ,
- McAllister G. D. ,
- Smith T. F.
-
↵
- Sparks K. A. ,
- Dieckmann C. L.
- ↵
-
↵
- Siepel A. ,
- Bejerano G. ,
- Pedersen J. S. ,
- Hinrichs A. S. ,
- Hou M. ,
- Rosenbloom K. ,
- Clawson H. ,
- Spieth J. ,
- Hillier L. W. ,
- Richards S. ,
- et al.
- ↵
- ↵
- ↵
-
↵
- Kampa D. ,
- Cheng J. ,
- Kapranov P. ,
- Yamanaka M. ,
- Brubaker S. ,
- Cawley S. ,
- Drenkow J. ,
- Piccolboni A. ,
- Bekiranov S. ,
- Helt G. ,
- et al.
- ↵
- ↵
-
↵
- Winzeler E. A. ,
- Shoemaker D. D. ,
- Astromoff A. ,
- Liang H. ,
- Anderson K. ,
- Andre B. ,
- Bangham R. ,
- Benito R. ,
- Boeke J. D. ,
- Bussey H. ,
- et al.
- ↵
-
↵
- Kumar M. ,
- Carmichael G. G.
-
↵
- Aravind L. ,
- Watanabe H. ,
- Lipman D. J. ,
- Koonin E. V.
- ↵
- ↵
- ↵
- ↵
-
↵
- Peterson J. A. ,
- Myers A. M.
- ↵
- ↵
-
↵
- Olivas W. M. ,
- Muhlrad D. ,
- Parker R.
-
↵
- McCutcheon J. P. ,
- Eddy S. R.
- ↵
- ↵
- ↵
-
↵
- R Development Core Team
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- Accumulation of noncoding RNA due to an RNase P defect in Saccharomyces cerevisiae
- Diverse environmental stresses elicit distinct responses at the level of pre-mRNA processing in yeast
- Insight Into the Mechanism of Nucleosome Reorganization From Histone Mutants That Suppress Defects in the FACT Histone Chaperone
- A yeast-based assay identifies drugs active against human mitochondrial disorders
- cis-Antisense RNA, Another Level of Gene Regulation in Bacteria
- Nucleosome Positioning in Saccharomyces cerevisiae
- 5'TRU: Identification and Analysis of Translationally Regulative 5'Untranslated Regions in Amino Acid Starved Yeast Cells
- Stable and dynamic nucleosome states during a meiotic developmental process
- The Paf1 Complex Represses ARG1 Transcription in Saccharomyces cerevisiae by Promoting Histone Modifications
- The nuclear RNA polymerase II surveillance system targets polymerase III transcripts
- Analysis of Affymetrix ChIP-Chip Data Using Starr and R/Bioconductor
- Regulated Antisense Transcription Controls Expression of Cell-Type-Specific Genes in Yeast
- Transcriptional Interference and Gene Orientation in Yeast: Noncoding RNA Connections
- RNAcode: Robust discrimination of coding and noncoding regions in comparative sequence data
- Execution of the meiotic noncoding RNA expression program and the onset of gametogenesis in yeast require the conserved exosome subunit Rrp6
- Antisense expression increases gene expression variability and locus interdependency
- Relicensing of Transcriptionally Inactivated Replication Origins in Budding Yeast
- RNA synthesis precision is regulated by preinitiation complex turnover
- Chromatin Remodeling around Nucleosome-Free Regions Leads to Repression of Noncoding RNA Transcription
- Structural and operational complexity of the Geobacter sulfurreducens genome
- Noncanonical transcript forms in yeast and their regulation during environmental stress
- Translational repression by PUF proteins in vitro
- Positioned and G/C-capped poly(dA:dT) tracts associate with the centers of nucleosome-free regions in yeast promoters
- A novel strategy of transcription regulation by intragenic nucleosome ordering
- Central Role of the Cell in Microbial Ecology
- A T9G Mutation in the Prototype TATA-Box TCACTATATATAG Determines Nucleosome Formation and Synergy with Upstream Activator Sequences in Plant Promoters
- Toggle involving cis-interfering noncoding RNAs controls variegated gene expression in yeast
- Pervasive transcription constitutes a new level of eukaryotic genome regulation
- An Overview of Nested Genes in Eukaryotic Genomes
- Transcription termination by nuclear RNA polymerases
- Ab initio construction of a eukaryotic transcriptome by massively parallel mRNA sequencing
- A canonical promoter organization of the transcription machinery and its regulators in the Saccharomyces genome
- TATA-binding Protein Variants That Bypass the Requirement for Mot1 in Vivo
- Prevalence of transcription promoters within archaeal operons and coding sequences
- LINE-Like Retrotransposition in Saccharomyces cerevisiae
- Evidence for a major role of antisense RNAs in cyanobacterial gene regulation
- Discovering structural cis-regulatory elements by modeling the behaviors of mRNAs
- Genome-wide allele- and strand-specific expression profiling
- Sequential Elimination of Major-Effect Contributors Identifies Additional Quantitative Trait Loci Conditioning High-Temperature Growth in Yeast
- Regulation of TATA-binding protein dynamics in living yeast cells
- Remodeling Yeast Gene Transcription by Activating the Ty1 Long Terminal Repeat Retrotransposon under Severe Adenine Deficiency
- Revisiting the Saccharomyces cerevisiae predicted ORFeome
- Unconventional Genomic Architecture in the Budding Yeast Saccharomyces cerevisiae Masks the Nested Antisense Gene NAG1
- A barrier nucleosome model for statistical positioning of nucleosomes throughout the yeast genome
- The Transcriptional Landscape of the Yeast Genome Defined by RNA Sequencing
- The Chemical Genomic Portrait of Yeast: Uncovering a Phenotype for All Genes
- Statistical Similarities between Transcriptomics and Quantitative Shotgun Proteomics Data
- Genome-wide discovery and verification of novel structured RNAs in Plasmodium falciparum
- Association of yeast Upf1p with direct substrates of the NMD pathway
- Trf4 targets ncRNAs from telomeric and rDNA spacer regions and functions in rDNA copy number control
- Mapping the C. elegans noncoding transcriptome with a whole-genome tiling microarray
- Yeast Mitochondrial Initiator tRNA Is Methylated at Guanosine 37 by the Trm5-encoded tRNA (Guanine-N1-)-methyltransferase
- Transcriptional interference: an unexpected layer of complexity in gene regulation
- The exosome and RNA quality control in the nucleus
- The DART classification of unannotated transcription within the ENCODE regions: Associating transcription with known and novel loci
- FAIRE (Formaldehyde-Assisted Isolation of Regulatory Elements) isolates active regulatory elements from human chromatin
- A role for noncoding transcription in activation of the yeast PHO5 gene
- A new paradigm for developmental biology
- Genome-wide identification of spliced introns using a tiling microarray