Calsyntenins Function as Synaptogenic Adhesion Molecules in Concert with Neurexins CELL REPORTS Um, J. W., Pramanik, G., Ko, J. S., Song, M., Lee, D., Kim, H., Park, K., Suedhof, T. C., Tabuchi, K., Ko, J. 2014; 6 (6): 1096-1109

Abstract

Multiple synaptic adhesion molecules govern synapse formation. Here, we propose calsyntenin-3/alcadein-β as a synapse organizer that specifically induces presynaptic differentiation in heterologous synapse-formation assays. Calsyntenin-3 (CST-3) is highly expressed during various postnatal periods of mouse brain development. The simultaneous knockdown of all three CSTs, but not CST-3 alone, decreases inhibitory, but not excitatory, synapse densities in cultured hippocampal neurons. Moreover, the knockdown of CSTs specifically reduces inhibitory synaptic transmission in vitro and in vivo. Remarkably, the loss of CSTs induces a concomitant decrease in neuron soma size in a non-cell-autonomous manner. Furthermore, α-neurexins (α-Nrxs) are components of a CST-3 complex involved in CST-3-mediated presynaptic differentiation. However, CST-3 does not directly bind to Nrxs. Viewed together, these data suggest that the three CSTs redundantly regulate inhibitory synapse formation, inhibitory synapse function, and neuron development in concert with Nrxs.

View details for DOI 10.1016/j.celrep.2014.02.010

View details for Web of Science ID 000333465000014

View details for PubMedID 24613359