A vacuum flask system is required to provide protection to the central building vacuum system or vacuum pump and to personnel who service the equipment. Figure 1 illustrates a proper set-up for handling liquid waste. Additionally, flasks A and B must be placed in secondary containment.
Figure 1. Liquid Waste. The left suction flask (A) is used to collect the contaminated fluids into a suitable decontamination solution; the right flask (B) serves as a fluid overflow collection vessel. An in-line HEPA filter (C) is used to protect the vacuum system (D) from microorganisms.
Stanford University has taken a strong stance against the use of gas burners or alcohol flames in biosafety cabinets. The use of such devices is not only extremely dangerous, but can also inactivate manufacturer’s warranties. There are many alternatives to the use of burners: microincinerator, disposable tissue culture supplies, etc. Please consult with the biosafety manager if you have any questions.
Installation and maintenance of BSCs
Installation of cabinets must be done by certified professionals. Stanford University has a contract with a certified company for installation, cabinet certification (must be done annually), decontamination and any other needs that may arise. Arrangements and payment for any of the above work must be scheduled by the PI or the department. For more information go to the Biosafety Cabinets subtopic.
