Heilshorn Biomaterials Group

Cai L., Dewi R., Goldstone A., Cohen J., Steele A., Woo J., Heilshorn SC. Regulating stem cell secretome using injectable hydrogels with in situ network formation. Advanced Healthcare Materials, 2016, 5(21):2758-2764 » PDF    » Article

Dubbin K., Hori Y., Lewis K., Heilshorn SC. Dual-stage crosslinking of a gel-phase bioink improves cell viability and homogeneity for 3D bioprinting. Advanced Healthcare Materials, 2016, 5(19): 2488-2492 » PDF    » Article
*This article was featured on the cover of Advanced Healthcare Materials

Parisi-Amon A., Lo D., Montoro D., Dewi R., Longaker M., Heilshorn SC. Protein-nanoparticle hydrogels that self-assemble in response to peptide-based molecular recognition. ACS Biomater. Sci. Eng., 2016, doi: 10.1021/acsbiomaterials.6b00286  » PDF    » Article

Madl CM, Heilshorn SC. Probing the metabolomics of stem cell differentiation with biomaterials. Chem, 2016, 1(2): 192-194  » PDF    » Article

Marquardt L., Heilshorn SC. Design of injectable materials to improve stem cell transplantation. Current Stem Cell Reports, 2016, 2(3):207-220  » PDF    » Article

Quarta M., Brett J., DiMarco R., De Morree A., Boutet S., Chacon R., Gibbons M., Garcia V., Su J., Shrager J., Heilshorn SC, Rando T. An artificial niche preserves the quiescence of muscle stem cells and enhances their therapeutic efficacy. Nature Biotechnology, 2016, 34:752–759  » PDF    » Article

Haugh M., Heilshorn SC. Integrating concepts of material mechanics, ligand chemistry, dimensionality and degradation to control differentiation of mesenchymal stem cells. Curr. Opin. Solid State Mater. Sci., 2016, 20(4):171-179  » PDF    » Article

Raphel J., Holodniy M., Goodman S.B., Heilshorn SC. Multifunctional coatings to simultaneously promote osseointegration and prevent infection of orthopaedic implants. Biomaterials, 2016, 84:301-314  » PDF    » Article

Madl CM, Katz L., Heilshorn SC. Bio-orthogonally crosslinked, engineered protein hydrogels with tunable mechanics and biochemistry for cell encapsulation. Advanced Functional Materials, 2016, 26(21):3612-3620  » PDF    » Article

Raphel J., Karlsson J., Galli S., Wennerberg A., Lindsay C., Haugh M., Pajarinen J., Goodman S.B., Jimbo R., Andersson M., Heilshorn SC. Engineered protein coatings to improve the osseointegration of dental and orthopaedic implants. Biomaterials, 2016, 83:269-282  » PDF    » Article

Benitez P., Mascharak S., Proctor A., Heilshorn SC. Use of protein-engineered fabrics to identify design rules for integrin ligand clustering in biomaterials. Integrative Biology, 2016, 8:50-61  » PDF    » Article

Shamloo A., Mohammadaliha N., Heilshorn SC., Bauer AL. A comparative study of collagen matrix density effect on endothelial sprout formation using experimental and computational approaches. Annals of Biomedical Engineering, 2015, Epub DOI: 10.1007/s10439-015-1416-2  » PDF    » Article

DiMarco R., Dewi R.E., Bernal G., Kuo C., Heilshorn S.C. Protein-engineered scaffolds for in vitro 3D culture of primary adult intestinal organoids. Biomaterials Science, 2015, 3:1376-85.  » PDF    » Article

Wang H., Heilshorn SC. Adaptable hydrogel networks with reversible linkages for tissue engineering. Advanced Materials, 2015, 27(25):3717-36.  » PDF    » Article

Ferreira MM., Dewi RE., Heilshorn SC. Microfluidic analysis of extracellular matrix-bFGF crosstalk on primary human myoblast chemoproliferation, chemokinesis, and chemotaxis. Integrative Biology, 2015, 7:569-579  » PDF    » Article

Madl CM., Heilshorn SC. Matrix interactions modulate neurotrophin-mediated neurite outgrowth and pathfinding. Neural Regeneration Research, 2015, 10(4):514-7.  » PDF    » Article

Cai L., Dewi RE., Heilshorn SC. Injectable hydrogels with in situ double network formation enhance retention of transplanted stem cells. Advanced Functional Materials, 2015, 25(9):1344-51.  » PDF    » Article

*Romano N., *Madl CM., Heilshorn SC. Matrix RGD ligand density and L1CAM-mediated Schwann cell interactions synergistically enhance neurite outgrowth. Acta Biomaterialia, 2015, 11(1):48–57.  » PDF    » Article
*Denotes co-first authorship.

Huggins KNL., Schoen AP., Arunagirinathan MA., Heilshorn SC. Multi-site functionalization of protein scaffolds for bimetallic nanoparticle templating. Advanced Functional Materials, 2014, 24(48):7737–44.  » PDF    » Article

Wang H., Cai L.,  Paul A., Enejder A., Heilshorn SC. Hybrid elastin-like polypeptide-polyethylene glycol (ELP-PEG) hydrogels with improved transparency and independent control of matrix mechanics and cell ligand density. Biomacromolecules, 2014, 15(9):3421–3428.     » Article       » PDF

Greenwood-Goodwin M., Teasley ES., Heilshorn SC. Dual-stage growth factor release within 3D protein-engineered hydrogel niches promotes adipogenesis. Biomaterials Science, 2014, 2:1627-1639.     » Article       » PDF
*This article was featured on the cover of Biomaterials Science

Romano N., Lampe K., Xu H., Ferreira M., Heilshorn SC. Microfluidic gradients reveal enhanced neurite outgrowth but impaired guidance within 3D matrices with high integrin ligand densities. Small, Epub 14 October 2014. DOI: 10.1002/smll.201401574.     » Article       » PDF

VanDersarl JJ., Mehraeen S., Schoen A., Heilshorn SC., Spakowitz A., Melosh NA. Rheology and simulation of 2-dimensional clathrin protein network assembly. Soft Matter, 2014,10:6219-6227.     » Article       » PDF

Chang DT., Chai R., DiMarco R., Heilshorn SC., Cheng A. Protein-engineered hydrogel encapsulation for 3D culture of murine cochlea. Otology & Neurotology, Epub 8 August 2014. DOI: 10.1097/MAO.0000000000000518.     » Article       » PDF

Mulyasasmita W., Cai L., Dewi R., Jha A., Ullmann SD., Luong RH., Huang NF., Heilshorn SC. Avidity-controlled hydrogels for injectable co-delivery of induced pluripotent stem cell-derived endothelial cells and growth factors. Journal of Controlled Release, 2014, 191:71-81.     » Article       » PDF

Xu H., Ferreira MM., Heilshorn SC. Small-molecule axon-polarization studies enabled by a shear-free microfluidic gradient generator. Lab on a Chip, 2014, 14(12): 2047-2056.     » Article       » PDF

McLaughlin LM., Xu H., Carden SE., Fisher S., Reyes M., Heilshorn SC., Monack DM. A microfluidic-based genetic screen to identify microbial virulence factors that inhibit dendritic cell migration. Integrative Biology, 2014, (6): 438-449.     » Article       » PDF

Madl CM., Mehta M., Duda GN., Heilshorn SC., Mooney DJ. Presentation of BMP-2 mimicking peptides in 3D hydrogels directs cell fate commitment in osteoblasts and mesenchymal stem cells. Biomacromolecules, 2014,15(2):445-55.     » Article       » PDF

Cai L., Dinh CB, Heilshorn SC. One-pot synthesis of elastin-like polypeptide hydrogels with grafted VEGF-mimetic peptides. Biomaterials Science, 2014, (2): 757-765.     » Article       » PDF

Cai L., Heilshorn SC. Designing ECM-mimetic materials using protein engineering. Acta Biomaterialia, 2014, 10(4): 1751-1760.     » Article       » PDF

DiMarco R, Su J, Yan K, Dewi R, Kuo CJ, Heilshorn SC. Engineering of three-dimensional microenvironments to promote contractile behavior in primary intestinal organoids. Integrative Biology, 2014, 6(2):127-142.     » Article       » PDF
*This article was featured on the cover of Integrative Biology

Mulyasasmita W, Cai L, Hori Y, Heilshorn SC. Avidity-controlled delivery of angiogenic peptides from injectable molecular-recognition hydrogels. Tissue Engineering, Part A, 2014, 20(15-16):2102-14.     » Article      » PDF

Schoen AP, Huggins KNL, Heilshorn SC. Engineered clathrin nanoreactors provide tunable control over gold nanoparticle synthesis and clustering. Journal of Materials Chemistry B, 2013, 1:6662-6669.     » Article      » PDF

Huang NF, Dewi RE, Okogbaa J, Lee JC, Rufaihah A, Heilshorn SC, Cooke JP. Chemotaxis of human induced pluripotent stem cell-derived endothelial cells. American Journal of Translational Research, 2013, 5(5):510-520.     » Article       » PDF

Shamloo A, Manchandia M, Ferreira M, Mani M, Nguyen C, Jahn T, Weinberg K, Heilshorn SC. Complex chemoattractive and chemorepellent Kit signals revealed by direct imaging of murine mast cells in microfluidic gradient chambers. Integrative Biology, 2013, 5:1076-1085.     » Article       » PDF

Chung C, Pruitt BL, Heilshorn SC. Spontaneous cardiomyocyte differentiation of mouse embryoid bodies regulated by hydrogel crosslink density. Biomaterials Science, 2013, 1(10): 1082-1090.     » Article       » PDF

Schoen AP, Cordella N, Mehraeen S, Arunagirinathan MA, Spakowitz AJ, Heilshorn SC. Dynamic remodeling of disordered protein aggregates is an alternative pathway to achieve robust self-assembly of nanostructures. Soft Matter, 2013, 9(38): 9137-9145.      » Article       » PDF

Schoen AP, Hommersom B, Heilshorn SC, Leunissen ME. Tuning colloidal association with specific peptide interactions. Soft Matter, 2013, 9(29): 6781-6785.     » Article       » PDF

Lampe KJ, Antaris AL, Heilshorn SC. Design of 3D engineered protein hydrogels for tailored control of neurite growth. Acta Biomaterialia, 2013, 9:5590-5599.     » Article       » PDF

Parisi-Amon A, Mulyasasmita W, Chung C, Heilshorn SC. Protein-engineered injectable hydrogel to improve retention of transplanted adipose-derived stem cells. Advanced Healthcare Materials, 2013, 2:428-432.     » Article   » PDF

Xu H, Heilshorn SC. Microfluidic investigation of BDNF enhanced neural stem cell chemotaxis in CXCL12 gradients. Small, 2013, 9:585-595.     » Article   » PDF

Benitez P, Sweet JA, Fink H, Chennazhi KP, Nair SK, Enejder A, Heilshorn SC. Sequence-specific crosslinking of electrospun elastin-like protein preserves bioactivity and native-like mechanics. Advanced Healthcare Materials, 2013, 2:114-118.     » Article   » PDF

Schoen AP., Schoen DT., Huggins KNL., Arunagirinathan MA., Heilshorn SC. Engineered protein templates synthesize inorganic nanomaterials. Chemical Engineering Progress, 2012, 108:47-50. 

Chung C, Lampe K, Heilshorn SC. Tetrakis (hydroxyl methyl) phosphonium chloride as a covalent crosslinking agent for cell encapsulation within protein-based hydrogels. Biomacromolecules, 2012, 13:3912-3916.     » Article       » PDF

Chung C, Anderson E, Reijo Pera R, Pruitt BL, Heilshorn SC. Hydrogel crosslinking density regulated temporal contractility of human embryonic stem cell-derived cardiomyocytes in 3D cultures. Soft Matter, 2012, 8:10141-10148.      » Article       » PDF

DiMarco RL, Heilshorn SC. Multifunctional materials through modular protein engineering. Advanced Materials, 2012, 24:3923-3940.      » Article       » PDF

Sengupta D, Gilbert P, Johnson K, Blau HM, Heilshorn SC. Protein-engineered biomaterials to generate human skeletal muscle mimics. Advanced Healthcare Materials, 2012, 1:785-789.      » Article   » PDF

Yan KS, Chia LA, Li X, Ootani A, Su J, Lee JY, Su N, Luo Y, Heilshorn SC, Amieva MR, Sangiorgi E, Capecchi MR, Kuo C. The intestinal stem cell markers Bmi1 and Lgr5 identify two functionally distinct populations. Proceedings of the National Academy of Sciences USA, 2012, 109:466-471.      » Article       » PDF

Raphel J, Parisi-Amon A, Heilshorn SC. Photoreactive elastin-like proteins for use as versatile bioactive materials and surface coatings. Journal of Materials Chemistry, 2012, 22:19429-19437.      » Article       » PDF

*This is a peer-reviewed, » Article for a special issue on Materials for Biosurfaces

Lampe KJ, Heilshorn SC. Building stem cell niches from the molecule up through engineered peptide materials. Accepted for publication, Neuroscience Letters, 2012, 519:138-146.      » Article       » PDF

*This is a peer-reviewed, invited» Article for a special issue on Bioengineered Approaches to Nervous System Repair

Aguado BA, Mulyasasmita W, Su J, Lampe KJ, Heilshorn SC. Improving viability of stem cells during syringe needle flow through the design of hydrogel cell carriers. Tissue Engineering Part A, 2012, 18:806-815.     » Article       » PDF

Shamloo A, Xu H, Heilshorn SC. Mechanisms of VEGF-induced path-finding by endothelial sprouts in biomaterials. Tissue Engineering Part A, 2012, 18:320-330.     » Article       » PDF

Schoen AP, Schoen DT, Huggins KN, Arunigirinathan MA, Heilshorn SC. Template engineering through epitope recognition: A modular, biomimetic strategy for inorganic nanomaterial synthesis. Journal of the American Chemical Society, 2011, 133:18202-18207.     » Article       » PDF

Mulyasasmita W, Lee JS, Heilshorn SC. Molecular-level engineering of protein physical hydrogels for predictive sol-gel phase behavior. Biomacromolecules, 2011, 12:3406-3411.     » Article       » PDF

Nevill JT, Mo A, Cord BJ, Palmer TD, Poo MM, Lee LP, Heilshorn SC. Vacuum soft lithography for one-step patterning of multiple biomolecules to direct neuronal polarization. Soft Matter, 2011, 7:343-347.     » Article       » PDF

*This article was featured on the cover of Soft Matter

Romano N, Sengupta D, Chung C, Heilshorn S. Protein-engineered biomaterials: Nanoscale mimics of the extracellular matrix. Biochimica et Biophysica Acta, 2011, 1810:339-349.     » Article       » PDF

*This is a peer-reviewed, invited» Article for a special issue on Emerging Applications of Nanotechnologies in Biomedicine

Kuhnert F, Mancuso MR, Shamloo A, Wang HT, Choksi V, Florek M, Su H, Fruttiger M, Young WL, Heilshorn SC, Kuo CJ. Essential regulation of CNS angiogenesis by the orphan G protein-coupled receptor GPR124. Science, 2010, 330:985-989.     » Article       » PDF

Schoen DT*, Schoen AP*, Hu L, Kim HS, Heilshorn SC, Cui Y. High speed water sterilization using one dimensional nanostructures. Nano Letters, 2010, 10:3628-3632.     » Article       » PDF

Shamloo A, Heilshorn SC. Matrix density mediates polarization and lumen formation of endothelial sprouts in VEGF gradients. Lab on a Chip, 2010, 10:3061-3068.     » Article       » PDF

*This article was featured on the cover of Lab on a Chip

Shelly M, Lim BK, Cancedda L, Heilshorn SC, Gao H, Poo MM. Local and long-range reciprocal regulation of cAMP and cGMP in axon/dendrite formation. Science, 2010, 327:547-552.     » Article       » PDF

Sengupta D, Heilshorn SC. Protein-engineered biomaterials: Highly tunable tissue engineering scaffolds. Tissue Engineering Part B: Reviews, 2010, 16:285-293.     » Article       » PDF

*This article was featured on the cover of Tissue Engineering Part B: Reviews

Straley KS, Wong Po Foo C, Heilshorn SC. Biomaterial design strategies for the treatment of spinal cord injuries. Journal of Neurotrauma, 2010, 27:1-19.     » Article       » PDF

Wong Po Foo C, Lee JS, Mulyasasmita W, Parisi-Amon A, Heilshorn SC. Two-component protein-engineered physical hydrogels for cell encapsulation. Proceedings of the National Academy of Sciences USA, 2009, 106:22067-22072.     » Article       » PDF

Straley KS and Heilshorn SC. Dynamic, three-dimensional pattern formation within enzyme-responsive hydrogels. Advanced Materials, 2009, 21:4148-4152.       » Press release     » Article       » PDF

*This article was featured on the cover of Advanced Materials

Straley KS and Heilshorn SC. Design and adsorption of modular engineered proteins to prepare customized, neuron-compatible coatings. Frontiers in Neuroengineering, 2009, 2:9.     » Article       » PDF

Wilson RJ, Hu W, Wong Po Foo C, Koh AL, Gaster RS, Earhart CM, Fu A, Heilshorn SC, Sinclair R, Wang SX. Formation and properties of magnetic chains for 100 nm nanoparticles used in separations of molecules and cells. Journal of Magnetism and Magnetic Materials, 2009, 321:1452-1458.     » Article       » PDF

Wang S, Wong Po Foo C, Warrier A, Poo MM, Heilshorn SC, Zhang X. Gradient lithography of engineered proteins to fabricate 2D and 3D cell culture environments. Biomedical Microdevices, 2009, 11:1127-1134.     » Article       » PDF

Straley K, Heilshorn SC. Independent tuning of multiple biomaterial properties using protein engineering.  Soft Matter, 2009, 5:114-124.     » Article       » PDF

Shamloo A, Ma N, Poo MM, Sohn L, Heilshorn SC. Endothelial cell polarization and chemotaxis in a microfluidic device.   Lab on a Chip, 2008, 8:1292-1299.     » Article       » PDF

Shelly M, Cancedda L, Heilshorn S, Sumbre G, Poo M.  LKB1/STRAD promotes axon initiation during neuronal polarization.  Cell, 2007, 129:565-577.     » Article       » PDF

Carrico IS, Maskarinec SA, Heilshorn SC, Mock ML, Liu JC, Nowatzki PJ, Franck C, Ravichandran G, Tirrell D.  Lithographic patterning of photoreactive cell-adhesive proteins.   Journal of the American Chemical Society, 2007, 129:4874-4875.     » Article       » PDF

Heilshorn SC,  Liu JC, DiZio KA, Tirrell DA.  Cell-binding domain context affects cell behavior on engineered proteins.  Biomacromolecules, 2005, 6:318-323.     » Article       » PDF

Liu JC, Heilshorn SC, Tirrell DA.  Comparative cell response to artificial extracellular matrix proteins containing the RGD and  CS5 cell-binding domains.  Biomacromolecules, 2004, 5:497-504.     » Article       » PDF

Heilshorn SC, DiZio KA, Welsh ER, Tirrell DA.  Endothelial cell adhesion to the fibronectin CS5 domain in artificial  extracellular matrix proteins.  Biomaterials, 2003, 24:4245-4252.     » Article       » PDF

Glenn RW, Sine MR, Evans MD, Carethers ME, Heilshorn SC "Liquid personal cleansing compositions which contain a  complex coacervate for improved sensory perception", U.S. patents #6,028,043 (2000), #5,932,528 (1999), #5,858,938 (1999),  #5,716,920 (1998). Assignee: The Procter & Gamble Company.

Benitez P, Heilshorn SC. (2013) "Microfluidic devices for quantifying the role of soluble gradients in early angiogenesis." Ed. Reinhart-King CA, Mechanical and Chemical Signaling in Angiogenesis, Heidelberg, Germany, Springer.

Raphel J, Parisi-Amon AP, Heilshorn SC. (2012) "Protein-Engineered Hydrogels." Eds. Taubert A, Mano J, Rodriquez-Cabello JC, Biomaterials Surface Science, Mannheim, Germany, Wiley-VCH Verlag.

Parisi-Amon A, Heilshorn SC. (2012) "Engineered Protein Biomaterials." Eds. Bronzino JD, Peterson DR, Section Eds. Fisher JP, Mikos AG, Biomedical Engineering Handbook, 4th Edition, Volume 4, Boca Raton, FL, CRC Press, Taylor & Francis Group.

Greenwood-Goodwin M, Heilshorn SC. (2012) "Hydrogels from Protein Engineering." Ed. Mano JF, Biomimetic Approaches for Biomaterials Development. Mannheim, Germany, Wiley-VCH Verlag.

Mulyasasmita W, Heilshorn SC. (2011) "Protein-Engineered Biomaterials: Synthesis and Characterization." Eds. Ducheyne P, Healy K, Hutmacher DW, Grainger DW, Kirkpatrick CJ, Comprehensive Biomaterials. Oxford, UK, Elsevier Science.

Wong Po Foo C, Heilshorn SC. (2010) "Protein Engineered Biomaterials." Eds. Park SJ, Cochran JR, Protein Engineering. Boca Raton, FL, CRC Press, Taylor & Francis Group.