Our Services

Donor Recruiting and Selection

• Recruit and schedule donors

Product Customization

• Irradiation
• Selection by ABO and/or CMV, per product
• Selection by Aspirin use
• Selection by demographic information (age, gender, ethnicity), per product.
• Special requests (e.g. red cell antigen, HLA type, repeat donor, matched tubes, etc.)

Clinical Laboratory Tests (Donor or product, unless otherwise specified)

• ABO/Rh
• CBC, automated, with 3 part differential
• CMV
• Hemoglobin S
• Hepatitis panel (incl. hepatitis B surface antigen, hepatitis B core (total) antibody, hepatitis C antibody, hepatitis B DNA, Hepatitis C RNA)
• HIV 1,2 antibody, HTLV-1,2 antibody, syphilis antibody, and HIV RNA
• T. cruzi antibody test
• West Nile RNA
• Infectious disease panel with counseling services
• Confirmatory/Supplemental testing (if infectious disease screen is positive)
• Hepatitis B Neutralization
• Hepatitis C 2^nd Manufacturer Screening Test
• HIV-1 IFA
• HIV-2 EIA
• Geenius HIV 1/2 Supplemental
• HTLV- Western Blot 2.4
• TPPA (Syphilis)
• RBC antibody screen
• White blood cell count, final product, flow cytometry

HLA Testing

• HLA typing, molecular, low resolution (loci: A, B, C, DR, DQ, DP)
• HLA typing, molecular, high resolution sequence analysis (loci: A, B, C, DR, DQ, DP)
• Typing of individual HLA locus upon request
• HLA antibody screening and/or identification: lgG Ab; C1q-binding Ab)
• Crossmatch testing, B and T cell
• Short tandem repeat (STR) analysis for chimerism or identity testing
• Subset isolation from bone marrow aspirate or peripheral blood (human CD34+, CD19+, CD56+, CD3+, CD15+ subsets) for chimerism analysis
• DNA extraction and storage
• Lymphocyte cryopreservation and storage
• Serum freezing and storage

Flow Cytometry

• Consultation on experimental design and panel design using Multiparameter flow cytometry
• Flow Cytometry analysis
• Multicolor Flow Cytometry analysis
• Flow Cytometry sorting
• Flow Cytometry sorting with cell prep
• T cell subset analysis
• Total lymphocyte subsets (TBNK)
• Cell isolation and cryopreservation
• Cytokine testing
• Immunophenotyping:
  • Total lymphocyte subsets (TBNK)
  • T cells and TH cell subsets
  • T regulatory cells
  • Total B cells and B cells subsets
  • Dendritics cells and subsets
  • Macrophages and Monocytes
  • NK cells
  • iNKT cells
  • Stem cell (CD34) enumeration

Instrumentation for FACS Analysis and FACS sorting:

• BD LSRII equipped with UV, violet, blue, yellow green and red lasers (18 parameters)
• BD FACSAria II equipped with violet, blue and red lasers  (14 parameters)
• BD FACSCantoII equipped with blue, red and violet lasers (10 parameters)

We offer a variety of testing, not limited to what is stated above. You may contact the Flow Cytometry supervisor for your specific needs. E-mail: lornat@stanford.edu