Imaging

Imaging

MRI, CT, PET, SPECT, luminescence and fluorescence whole animal imaging, ultrasound, live animal and tissue-slice two-photon confocal microscopy, inverted microscope confocal imaging, deconvolution widefield microscopy, SEM, TEM.

Shared Facilities:

Cell Sciences Imaging Facility (CSIF)

• Description — Microscopy: advanced, high resolution light and electron microscopy services. Confocal, multi-photon, and deconvolution wide-field microscopy for live cell/tissue and fixed samples. Transmission (TEM) and scanning electron microscopy (SEM) imaging and EM sample preparation.
• Location — Beckman B050, B051, B001
• Questions — Contact Jon Mullholland  , (650) 725-7532
• Website — http://microscopy.stanford.edu
• Selected References

  Adipose tissue-derived stem cells display a proangiogenic phenotype on 3D scaffolds. Neofytou EA, Chang E, Patlola B, Joubert LM, Rajadas J, Gambhir SS, Cheng Z, Robbins RC, Beygui RE. J Biomed Mater Res A. 2011 Sep 1;98(3):383-93. doi: 10.1002/jbm.a.33113. Epub 2011 May 31. PubMedID: 21630430.

  Entrapment of viral capsids in nuclear PML cages is an intrinsic antiviral host defense against varicella-zoster virus. Reichelt M, Wang L, Sommer M, Perrino J, Nour AM, Sen N, Baiker A, Zerboni L, Arvin AM. PLoS Pathog. 2011 Feb 3;7(2):e1001266.PubMedID: 21304940; PMCID: PMC3033373.

  Differential Localization and Independent Acquisition of the H3K9me2 and H3K9me3 Chromatin Modifications in the Caenorhabditis elegans Adult Germ Line. Bessler JB, Andersen EC, Villeneuve AM (2010) PLoS Genet 6(1): e1000830. doi:10.1371/journal.pgen.1000830 PubMedID: 20107519; PMCID: PMC2809760.

Lucas Center for Imaging

• Description — Apply medical imaging technology to fundamental physiologic and patho-physiologic studies involving humans and animal models. Advance medical imaging technology to improve health and patient care. Provide educational opportunities to researchers, clinicians, and students. Serve the academic and industrial community. Whole Body Imaging for humans and animals models. Equipment includes: Three 3.0 Tesla MRI systems, 7.0 Tesla MRI system and Fluoro Suite.
• Location — Lucas Center
• Questions — Contact Anne Sawyer  , (650) 725-9697
• Website — http://rsl.stanford.edu/lucas/
• Selected References

  Associative Retrieval Processes in the Human Medial Temporal Lobe: Hippocampal Retrieval Success and CA1 Mismatch Detection. Chen A; Olsen RK; Preston AR; Glover GH; Wagner AD. Learning & Memory 18:523-8 (2011). PubMedID: 21775513.

  Neural Systems Predicting Long-Term Outcome in Dyslexia. Hoeft F, McCandliss B, Black J, Gantman A, Zakerani N, Hulme C, Lyytinen H, Whitfield-Gabrieli S, Glover GH, Reiss AL, Gabrieli JDEPNAS 108:361-6 (2011). PubMedID: 21173250; PMCID: PMC3017159.

  Modulation of Subgenual Anterior Cingulate Cortex Activity with Real-Time Neurofeedback. Hamilton JP, Glover GH, Hsu JJ, Johnson RF, Gotlib IH. Hum. Brain Map. 32:22-31 (2011). PubMedID: 21157877; PMCID: PMC3049174.

  A quantitative comparison of NIRS and fMRI across multiple cognitive tasks. Cui X, Bray S, Bryant DM, Glover GH, Reiss AL. NeuroImage 54:2808-14 (2010). PubMedID: 21047559; PMCID: PMC3021967.

Neuroscience Microscopy Service

• Description — 2-photon microscopy: both in vivo and tissue slice imaging and photoactivation. Confocal microscopy: time-lapse confocal imaging with environmental control, spectral imaging, and standard fixed-tissue imaging. Fluorescence microscopy: array tomography data acquisition and analysis. Image analysis: high-end, dual quad-core image analysis workstations with 24 GB memory and 3D graphics cards, Imaris (3D visualization and rendering), NeuroLucida (process tracing and quantification), Matlab (general number-crunching, image analysis) and ImageJ (general biological image analysis) software packages.
• Location — Lokey Stem Cell Building (SIM1), Suite G0901
• Questions — Contact Andrew Olson, PhD  , (650) 723-8818
• Website — http://nisms.stanford.edu
• Selected References

  Versatile synthesis and rational design of caged morpholinos. Ouyang X, Shestopalov IA, Sinha S, Zheng G, Pitt CL, Li WH, Olson AJ, Chen JK J Am Chem Soc. 2009 Sep 23;131(37):13255-69. PubMedID: 19708646; PMCID: PMC2745229.

  Using induced pluripotent stem cells to investigate cardiac phenotypes in Timothy syndrome. Yazawa M, Hsueh B, Jia X, Pasca AM, Bernstein JA, Hallmayer J, Dolmetsch RE. Nature. 2011 Mar 10;471(7337):230-4. Epub 2011 Feb 9. PubMedID: 21307850; PMCID: PMC3077925.

  An antibody against SSEA-5 glycan on human pluripotent stem cells enables removal of teratoma-forming cells. Tang C, Lee AS, Volkmer JP, Sahoo D, Nag D, Mosley AR, Inlay MA, Ardehali R, Chavez SL, Pera RR, Behr B, Wu JC, Weissman IL, Drukker M. Nat Biotechnol. 2011 Aug 14;29(9):829-34. PubMedID: 21841799.

Stanford Center for Cognitive and Neurobiological Imaging (CNI)

• Description — The Stanford Center for Cognitive and Neurobiological Imaging (CNI) is a shared facility, dedicated to research and teaching. The Center provides resources for researchers and students used in cognitive and neurobiological sciences.
  Discoveries about the brain have implications for fields ranging from Business, Law, Psychology, and Education. The Stanford Center for Cognitive and Neurobiological Imaging (CNI) supports scientific investigations into the brain that make rigorous connections between neuroscience and society.
• Location — 061 Jordan Hall, Building 420, 450 Serra Mall
• Questions — Contact Laima Baltusis  , (650) 725-8382
• Website — http://cni.stanford.edu/

Stanford Center for Innovation and In Vivo Imaging (SCi3)

• Description — The Stanford Center for Innovation in In Vivo Imaging (Sci^3) is a small animal imaging facility that allows non-invasive studies on animals such as mice and rats. In addition to instruments routinely used in the clinic, such as ultrasound, microCT, microPET, microSPECT/CT and MRI (each optimized for animal research), the facility also has instruments to investigate bio-distribution of molecular imaging probes such as GFP, fluorescent markers and bioluminescent proteins in these animals. Access to surgical benches and supplies is available. Instrument training is provided for free during regular sessions, which are posted through an email service. In addition, full computer support and data archiving is provided to investigators.
• Location — Clark Center S040
• Questions — Contact Tim Doyle, PhD  , (650) 724-8250
• Website — http://sci3.stanford.edu/
• Selected References

  Substrate elasticity regulates skeletal muscle stem cell self-renewal in culture. Gilbert PM, Havenstrite KL, Magnusson KE, et al. Science; 329(5995): p. 1078-81. PubMedID: 20647425; PMCID: PMC2929271

  Global and local fMRI signals driven by neurons defined optogenetically by type and wiring. Lee JH, Durand R, Gradinaru V, et al. Nature; 465(7299): p. 788-92. PubMedID: 2047328; PMCID: PMC3177305

  Fgf-9 is required for angiogenesis and osteogenesis in long bone repair.  Behr B, Leucht P, Longaker MT, et al. Proc Natl Acad Sci U S A; 107(26): p. 11853-8. PubMedID: 20547837; PMCID: PMC2900703.