Mass Spectrometers
Thermo Orbitrap Elite with Electron Transfer Dissociation (ETD) and Thermo NanoMate 3000 UHPLC
The Thermo Scientific Orbitrap Elite hybrid ion trap-Orbitrap mass spectrometer provides the highest resolving power of all Orbitrap-based instruments of up to 240,000 FWHM at a scan rate of 4 Hz, to achieve ultimate detection limits and selectivity in the full-scan MS for analysis of complex samples and providing accurate quantitation results. MS/MS in the ion trap allows probing the least-abundant components in a complex mixture. Fast, high-quality MS and MS/MS scans allow maximal identification of peptides in a complex matrix. Additionally, multiple fragmentation techniques, including collision-induced dissociation (CID), higher-energy collisional dissociation (HCD) and electron transfer dissociation (ETD), provide complementary fragment information for maximal sequence coverage.
Specifications*
Mass Range Dynamic Range MS Scan Power Mass Accuracy Resolution 50 to 2000 m/z; 200 to 4000 m/z > 5000 within a single scan MSn for n = 1 through 10 < 3ppm RMS with external calibration < 1ppm RMS using internal calibration 60,000 at m/z 400 at a scan rate of 4Hz Minimum resolution 15,000 at m/z 400 Maximum resolution > 240,000 at m/z 400 *Thermo Scientific Orbitrap Elite Product Specifications #PS30229 (05/11)
Thermo LTQ Orbitrap Velos with Eksigent NanoLC-2D HPLC
The Thermo Scientific LTQ Orbitrap Velos mass spectrometer combines the mass accuracy and ultra-high resolution of the Orbitrap mass analyzer with resolving power of up to 100,000 FWHM at a scan rate of 1 Hz, with the increased sensitivity and improved cycle time of the LTQ Velos dual-pressure linear ion trap, to achieve excellent detection limits and selectivity in the full-scan MS for analysis of complex samples and providing accurate quantitation results. MS/MS in the ion trap allows probing the least-abundant components in a complex mixture and high-quality MS and MS/MS scans allow maximal identification of peptides in a complex matrix.
Specifications*
Mass Range Dynamic Range MS Scan Power Mass Accuracy Resolution 50 to 2000 m/z; 200 to 4000 m/z > 5000 within a single scan MSn for n = 1 through 10 < 3ppm RMS with external calibration < 1ppm RMS using internal calibration 60,000 at m/z 400 at a scan rate of 1Hz Minimum resolution 7500 at m/z 400 Maximum resolution > 100,000 at m/z 400 *Thermo Scientific LTQ Orbitrap Velos Product Specifications #PS30185 (05/09)
AB SCIEX 5800 TOF/TOF System
The AB SCIEX 5800 TOF/TOF system provides a confident path to protein identification and relative quantitation for occasional users who wish to acquire proteomics data to complement other approaches (genomics, metabolomics, etc.). The system's speed and sensitivity make it ideal for analysis of intact proteins purified by electrophoresis and column chromatography as well as relative quantitation of peptides in a digest using iTRAQ isobaric tagging. Electrospray ionization (ESI) and MALDI have been shown to provide complementary information for peptide identification. MALDI TOF/TOF data can be used to complement ESI data for a more in-depth identification and quantitation of proteins. Furthermore, the LC-MALDI workflow separates the time constraints of liquid chromatography (LC) from the mass spectrometry. With your chromatographic run "stored" on a MALDI plate, enabling subsequent re-analysis, and the 5800 TOF/TOF system automatically spends the time required to get the maximum information from any time point in the chromatographic separation.
Specifications*
Laser Repetition Rate Mass Range Mass Accuracy Resolution Resolution (broad mass range) MS, 200 and 400 Hz; MS/MS, 200, 400 and 1000 Hz 100 to 250,000 m/z (up to 1.2 MDa with COVALX HM2 Detector) < 10 ppm RMS with external calibration < 2.5 ppm RMS using internal calibration 30,000 for single peak in mass range 1200 to 3700 m/z > 16,000 for Angiotensin 1 > 18,000 for Glu1-Fib B > 25,000 for ACTH 1-17 and ACTH 18-39 > 18,000 for ACTH 7-38 *Provided by AB SCIEX (unpublished)
COVALX HM2 High Mass Detector
The AB SCIEX 5800 TOF/TOF system has been fitted with a COVALX HM2 High-Mass Detector that enables detection of macromolecular ions up to 1.2 megadaltons (MDa) with nM sensitivity. The HM2 high-mass detector retrofit does not alter any of the features of the MALDI instrument, and both the standard linear and reflectron detectors remain available. The high-mass detector supports a number of important applications including
- intact protein complex analysis,
- antibody characterization, and
- therapeutic protein aggregation analysis.
AB SCIEX Tempo LC-MALDI Spotting System
The Tempo LC-MALDI Spotting System provides a high precision plate spotting technology. It provides contact-free electrostatic deposition of droplets and includes a three-channel micro-LC pump for the LC gradient and post-column addition of MALDI matrix. For greater throughput and automation, the system accommodates multiple plates and multiple LC runs per plate. An integrated autosampler and low-dispersion UV detector are also included. LC-MALDI frees MS analysis from the time constraints of LC to enable more in-depth interrogation of samples, which can be of particular advantage in applications involving intact protein separations as well as analysis of tryptic digests. The Tempo LC-MALDI system dispenses droplets on plates by applying a high voltage electrostatic pulse on the sample needle at controlled intervals. This non-contact deposition technique virtually eliminates any occurrence of missed or misaligned spots and routinely delivers highly reproducible results and high-quality MS data. Spot coordinates are transferred directly to the AB SCIEX 5800 TOF/TOF system to facilitate mass spectrometry.
SimulTOF 200 Combo MALDI-TOF
The SimulTOF 200 Combo mass spectrometer is the highest performance bench top reflector system available. It is suited for intact protein, peptide and small molecule analysis. It is particularly well suited for imaging mass spectrometry (IMS). A high performance hybrid linear detector provides excellent mass range (100 Da to 500 kDa), speed (5 kHz, up to 100 spectra/sec) and dynamic range. A second high performance microchannel plate detector is used in the reflector mode. The SimulTOF 200 Combo was designed for ease-of-use through intuitive software, and low maintenance by exploiting new concepts in instrument design. The plate editor allows the user to specify the geometry of any plate the system might support including conventional plates as well as microscope slides for IMS.
Specifications*
20 kV source and novel high speed, high mass detector provides very high sensitivity, resolving power, and accuracy over a broad mass range. High laser rate (5 kHz) and high acquisition rate (up to 100 spectra/sec) makes tissue imaging practical. Resolving power in Linear Mode: >6000 for ACTH @ 2465 Da; Resolving power in Reflector Mode: >20,000 for Neurotensin @ 1672.92 Da and >10,000 for full range of from 1000 Da to 1 kDa. Mass range: 100 Da to 500 kDa. Sensitivity, Linear Mode: S/N>10 for ACTH (2465 Da) @ 1 attomole/mL and S/N>10 for IgG (150,000 Da) @ 1 fmole/mL. Sensitivity, Reflector Mode: S/N>10 for ACTH (2465 Da) @ 1 fmole/mL. Mass Accuracy, Reflector Mode: <5ppm across the sample calibrating on a single spot.
Agilent Technologies 6490 Triple Quadrupole LC/MS
Incorporating breakthrough iFunnel technology, the Agilent 6490 Triple Quadrupole LC/MS takes detection limits lower than ever. Users can achieve zeptomole level sensitivity and an unprecedented six orders of linearity for the most demanding quantitative analyses. Proprietary iFunnel technology dramatically increases ion sampling and transmission efficiency, enabling the lowest limits of detection and quantitation. Triggered MRM combines fast and sensitive MRM quantitation with the generation of a product ion spectrum for library searching, compound screening and confirmation. MassHunter Optimizer software automatically optimizes MRM transitions and fragmentor and collision energies for both small molecules and peptides. Ultra fast MRM acquisition speeds and ion polarity switching, an innovative collision cell eliminates cross-talk and enables 1ms MRM dwell times, together with fast polarity switching, ensures compatibility with UHPLC analyses. Dynamic MRM simplifies method development and allows thousands of compounds to be quantified in a single method with optimal sensitivity and reproducibility. Comprehensive and easy-to-use MassHunter Workstation software dramatically enhances productivity from method optimization, data acquisition to data processing and reporting.
Agilent 1260 Infinity HPLC-Chip Cube MS Interface
The Agilent 6490 Triple Quadrupole LC/MS may be configured with the Agilent 1260 Infinity HPLC-Chip Cube MS Interface, a microfluidic chip-based technology specifically designed for nanoelectrospray LC/MS. Based on Agilents laser etched polyimide HPLC-Chip and the HPLC-Chip Cube MS interface, the Agilent HPLC-Chip/MS system affords nanoelectrospray MS sensitivity, robustness, reliability, and ease of use. Reusable HPLC-Chips seamlessly integrate sample enrichment and separation nanocolumns, tubing, connections, and spray needle of a traditional nanoelectrospray LC/MS system into a biocompatible polymer chip. This results in narrower, better-defined peaks due to elimination of post-column dead volumes. HPLC-Chip Cube provides easy to use operation with automatic chip loading, solvent and sample delivery to the chip, high pressure switching of flows and automated chip positioning in the MS source. In the Proteomics Core, the HPLC-Chip Cube MS interface is used with Agilent 1260 Infinity series nanoflow and capillary pumps. When combined with the Agilent 6490 Triple Quadrupole MS, the HPLC-Chip for LC/MS offers even greater overall reliability and ease-of-use for many LC applications. HPLC-Chips with novel surface chemistries are currently available for protein ID, biomarker discovery and verification, phosphopeptide analysis, glycan analysis, and intact protein analysis.
Agilent 1290 Infinity Series UHPLC
The Agilent 6490 Triple Quadrupole LC/MS may be configured with the Agilent 1290 Infinity Series UHPLC (ultrahigh performance liquid chromatograph). The Agilent 1290 is designed to provide highest speed, resolution and sensitivity. The infinite power range that combines ultra high pressure up to 1200 bar and high flow rates up to 5 mL/min for maximum chromatographic performance, compatibility, and flexibility allows enables the user to deploy any particle type, any column dimensions, or any mobile and stationary phases. Innovative technology components offer excellent performance for both UHPLC and HPLC applications. The 1290 Infinity Series UHPLC is the first system that provides the foundation for method transfer to or from any Agilent or non-Agilent UHPLC or HPLC system. Lab Advisor software with intuitive diagnostic and monitor capabilities and alert functions notifies users of problems.
