Department of Plant Biology

Carnegie Institution for Science

260 Panama Street

Stanford, CA 94305

Phone: (650) 325-1521 x261

Fax: (650) 325-6857 

Email: ehrhardt stanford.edu

Plant Cell Development
We investigate plant cell development and morphogenesis, utilizing live cell imaging techniques and moelcular genetics  to visualize and investigate the organization and dynamic behaviors of molecules and organelles. Problems of special interest include how cells generate asymmetries and specific shapes. A current focus is how the cortical microtubule cytoskeleton acquires organization and how this organization in turn functions to organize the plasma membrane and cell wall to guide patterns of cell growth and division.

 

Selected Publications:

 

Nakamura M, Ehrhardt DW1, Hashimoto T1. (2010) Microtubule and katanin dependent dynamics of microtubule nucleation complexes in the acentrosomal Arabidopsis cortical array. Nature Cell Biol. Published ahead of print October 11, 2010. doi 038/ncb2110.

 

Chen S, Ehrhardt DW, Somerville CR  (2010) Mutations of cellulose synthase (CESA1) phosphorylation sites modulate anisotropic cell expansion and bidirectional mobility of cellulose synthase. Proc Natl Acad Sci U S A. 2010 Oct 5;107(40):17188-93. Epub 2010 Sep 20.

 

Opportunities to explore plant membrane organization with super-resolution microscopy. (2010) Gutierrez R, Grossmann G, Frommer WB, Ehrhardt DW. Plant Physiol. 2010 Oct;154(2):463-6.

 

Gutierrez R, Lindeboom J, Paredez AR, Emons AM and Ehrhardt DW. (2009) The microtubule cortical array organizes cellulose synthase trafficking. Nat Cell Biol. 2009 Jul;11(7):797-806. Epub 2009 Jun 14.

 

Paredez AR, Persson S, Ehrhardt DW, Somerville CR. (2008) Genetic evidence that cellulose synthase activity influences microtubule cortical array organization.  Plant Physiol. 2008 Aug;147(4):1723-34. Epub 2008 Jun 26.

 

Ying-Lang Wan, William Eisinger, David Ehrhardt, Ulrich Kubitscheck, Frantisek Baluska, and Winslow Briggs (2008) The Subcellular Localization and Blue-Light-Induced Movement of Phototropin 1-GFP in Etiolated Seedlings of Arabidopsis thaliana. Mol Plant 1: 103-117.

 

Lalonde S, Ehrhardt DW, Loqué D, Chen J, Rhee SY, Frommer WB (2008) Molecular and cellular approaches for the detection of protein-protein interactions: latest techniques and current limitations. Plant J. 53(4):610-35.

 

Ehrhardt DW (2008) Straighten up and fly right: microtubule dynamics and organization of non-centrosomal arrays in higher plants. Curr Opin Cell Biol. 20(1):107-16.

 

Kirik V, Herrmann U, Parupalli C, Sedbrook JC, Ehrhardt* DW, Hülskamp* M (2007) CLASP localizes in two discrete patterns on cortical microtubules and is required for cell morphogenesis and cell division in Arabidopsis. J Cell Sci. 120(Pt 24):4416-25. Epub 2007 Nov 27. *co-PI’s.

 

Silady RA, Ehrhardt DW, Jackson K, Faulkner C, Oparka K, Somerville CR. (2007) The GRV2/RME-8 protein of Arabidopsis functions in the late endocytic pathway and is required for vacuolar membrane flow. Plant J. 53(1):29-41. Epub 2007 Oct 27.

 

Walker KL, Müller S, Moss D, Ehrhardt DW, Smith LG (2007) Arabidopsis TANGLED identifies the division plane throughout mitosis and cytokinesis. Curr Biol. 17(21):1827-36. Epub 2007 Oct 25.

 

DeBolt S, Gutierrez R, Ehrhardt DW, Somerville C. (2007) Nonmotile cellulose synthase subunits repeatedly accumulate within localized regions at the plasma membrane in Arabidopsis hypocotyl cells following 2,6-dichlorobenzonitrile treatment. Plant Physiol. 145(2):334-8.

 

DeBolt S., Gutierrez R., Ehrhardt D.W., Melo C.V., Ross L., Cutler S.R., Somerville C.R., and Bonetta, D. (2007) Morlin, an inhibitor of cortical microtubule dynamics and cellulose synthase movement. PNAS. published March 27, 2007, 10.1073/pnas.0700789104

 

Paredez, A, Wright A.,  Ehrhardt D.W. (2006) Microtubule cortical array organization and plant cell morphogenesis. Curr Opin Plant Biol. 2006 Dec;9(6):571-8. Epub 2006 Sep 28.

 

Paredez, A., Somerville CR, Ehrhardt DW. (2006) Dynamic visualization of cellulose synthase demonstrates functional association with cortical microtubules. Science. Published online April 20 2006; 10.1126/science.1126551.

 

Shaw SR, Ehrhardt DW. (2006) Microtubule dynamics and organization in the plant cortical array. Annu Rev Plant Biol. 2006;57:859-75.

 

Koh S, Andre A, Edwards H, Ehrhardt D, Somerville S. (2005) Arabidopsis thaliana subcellular responses to compatible Erysiphe cichoracearum infections. Plant J. 44(3):516-29.

 

Lalonde S, Ehrhardt DW, Frommer WB. (2005) Shining light on signaling and metabolic networks by genetically encoded biosensors. Curr Opin Plant Biol. 8(6):574-81. Epub 2005 Sep 26.

 

Assaad FF, Qiu JL, Youngs H, Ehrhardt D, Zimmerli L, Kalde M, Wanner G, Peck SC, Edwards H, Ramonell K, Somerville CR, Thordal-Christensen H. (2004) The PEN1 syntaxin defines a novel cellular compartment upon fungal attack and is required for the timely assembly of papillae. Mol Biol Cell. 15(11):5118-29.

 

Sedbrook JC, Ehrhardt DW, Fisher SE, Scheible WR, Somerville CR. (2004) The Arabidopsis sku6/spiral1 gene encodes a plus end-localized microtubule-interacting protein involved in directional cell expansion. Plant Cell. 16:1506-20. Epub 2004 May 21.

 

Tian GW, Mohanty A, Chary SN, Li S, Paap B, Drakakaki G, Kopec CD, Li J, Ehrhardt D, Jackson D, Rhee SY, Raikhel NV, Citovsky V. (2004) High-throughput fluorescent tagging of full-length Arabidopsis gene products in planta. Plant Physiol. 2004 135:25-38.

 

Shaw S.L., Kamyar R. and Ehrhardt D.W. (2003) Sustained Microtubule Treadmilling in Arabidopsis Cortical Arrays.  Science. Published online 24 April 2003; 10.1126/science.1083529.

 

Ehrhardt D. (2003) GFP technology for live cell imaging. Curr Opin Plant Biol. 2003 6:622-8

 

Cutler S.R. and Ehrhardt D.W. (2002) Polarized cytokinesis in vacuolate cells of Arabidopsis.. Proc. Natl. Acad. Sci. 99: 2812-2817.

 

Cutler S.R., Ehrhardt D.W. (2000) Dead cells don't dance: insights from live-cell imaging in plants. Curr Opin Plant Biol. 2000 Dec;3(6):532-537.

 

Cutler S.R., Ehrhardt D.W., Somerville C.R. (2000) Random GFP::cDNA fusions enable visualization of subcellular structures in cells of Arabidopsis at a high frequency. Proc. Natl. Acad. Sci. 97:3718-3723.